Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606164109_6jl6 TGATCAGTCAAGTGAATTTGAAAAACTTTGAG(+AAAAAA)GTACCATCTGTAACATCTCTCATGTTAGCCT NGG 10 8 17 8 24 0 100 5 10
    240606164108_b67c TTCCAAGGCTAACATGAGAGATGTTACAGATG(+AAAAAA)GTACCTCAAAGTTTTTCAAATTCACTTGACT NGG 10 8 17 8 24 0 100 5 10
    240606164107_1t6x TGATCAGTCAAGTGAATTTGAAAAACTTTGAG(+AAAAAA)GTACCATCTGTAACATCTCTCATGTTAGCCT NGG 10 8 17 8 24 0 100 5 10
    240606164106_wx83 TTCCAAGGCTAACATGAGAGATGTTACAGATG(+AAAAAA)GTACCTCAAAGTTTTTCAAATTCACTTGACT NGG 10 8 17 8 24 0 100 5 10
    240606164104_r1ls TGATCAGTCAAGTGAATTTGAAAAACTTTGAG(+AAAAAA)GTACCATCTGTAACATCTCTCATGTTAGCCT NGG 10 8 17 8 24 0 100 5 10
    240606164103_obew ATGTATGTTTTGCCTGCCACAACCCGTACCTG(+AAAAAA)GTACCAGCAAAACCTGCAAAAAGCAAACAAG NGG 10 8 17 8 24 0 100 5 10
    240606164101_c3v9 CCTGGCTTGTTTGCTTTTTGCAGGTTTTGCTG(+AAAAAA)GTACCAGGTACGGGTTGTGGCAGGCAAAACA NGG 10 8 17 8 24 0 100 5 10
    240606164100_w51l AGACTCATTAATAGGATTGTTAGCAACCACTG(+AAAAAA)GATCCTCTGGTGGATAGACAAATGTTCTATA NGG 10 8 17 8 24 0 100 5 10
    240606164058_rnl8 TTCCATATAGAACATTTGTCTATCCACCAGAG(+AAAAAA)GATCCAGTGGTTGCTAACAATCCTATTAATG NGG 10 8 17 8 24 0 100 5 10
    240606164056_tqwh ATGAGATGAAATATTTGCTTCGCACACGCAGG(+AAAAAA)GATCCCACTATAGAAGTTCTCTTCAAAGGAG NGG 10 8 17 8 24 0 100 5 10