Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606162949_igol TCACAACTAAGGTTACCCCCATTGATTCTTGG(+AAAAAA)GTACCTCCCCTATCCCATACCTCCCTCATGT NGG 10 8 17 8 24 0 100 5 10
    240606162947_9svw TGAAGACATGAGGGAGGTATGGGATAGGGGAG(+AAAAAA)GTACCCAAGAATCAATGGGGGTAACCTTAGT NGG 10 8 17 8 24 0 100 5 10
    240606162945_uymd GTCAGGCCTTTCACACCAATACTCCCCTCCTG(+AAAAAA)GTACCAACTTCTTAGGTTGGGTTTTATCACC NGG 10 8 17 8 24 0 100 5 10
    240606162944_qzzl CTTCAGGTGATAAAACCCAACCTAAGAAGTTG(+AAAAAA)GTACCAGGAGGGGAGTATTGGTGTGAAAGGC NGG 10 8 17 8 24 0 100 5 10
    240606162942_yvuv GGTACTGTTAAGACCCACACCTGTGGCTCTCG(+AAAAAA)GTACCTTTTGGGGAGGTGAGGCAGAGCCGAA NGG 10 8 17 8 24 0 100 5 10
    240606162941_aalw TATTTTTCGGCTCTGCCTCACCTCCCCAAAAG(+AAAAAA)GTACCGAGAGCCACAGGTGTGGGTCTTAACA NGG 10 8 17 8 24 0 100 5 10
    240606162940_wb3r TCAGCAGTTTAGGGAGGACTGTGCAAAGGAAG(+AAAAAA)GTACCTTTTTGAACCACAACAGGTGTTTGCC NGG 10 8 17 8 24 0 100 5 10
    240606162938_78op ACACAGGCAAACACCTGTTGTGGTTCAAAAAG(+AAAAAA)GTACCTTCCTTTGCACAGTCCTCCCTAAACT NGG 10 8 17 8 24 0 100 5 10
    240606162937_qkl4 TGTGACCAATTATATGGTCAGTTTTGGAGAAG(+AAAAAA)GTACCATAAGGTGCTGAGAAGAAGGTATATC NGG 10 8 17 8 24 0 100 5 10
    240606162936_g99s AAAAGGATATACCTTCTTCTCAGCACCTTATG(+AAAAAA)GTACCTTCTCCAAAACTGACCATATAATTGG NGG 10 8 17 8 24 0 100 5 10