Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606163027_g7a8 GAAGGGGAAGGGCTGCAGTCAGAAAGTGCGTG(+AAAAAA)GTACCTTCCTGCAAGCACACGCTTCTTGTCA NGG 10 8 17 8 24 0 100 5 10
    240606163025_i86o ATGGTTCGTATACTACATCACTTGCTGATAGG(+AAAAAA)GTACCTCAGAGTGCCCTAGAAACCACACGAC NGG 10 8 17 8 24 0 100 5 10
    240606163024_4k2o AAGAAGTCGTGTGGTTTCTAGGGCACTCTGAG(+AAAAAA)GTACCCTATCAGCAAGTGATGTAGTATACGA NGG 10 8 17 8 24 0 100 5 10
    240606163023_6wg7 CTTGCAAGTTATGAAAATAAAGTATATAAAAG(+AAAAAA)GATCCTGTGCTATTGTGTGCGTGGTTGCTGA NGG 10 8 17 8 24 0 100 5 10
    240606163021_yed7 TTGTCTCAGCAACCACGCACACAATAGCACAG(+AAAAAA)GATCCTTTTATATACTTTATTTTCATAACTT NGG 10 8 17 8 24 0 100 5 10
    240606163020_apx8 CCTAGGCTGTGGACCTGGGCTCTATAAGAAAG(+AAAAAA)GTACCTGAAAATGAGACTAGGAATGAGCCAG NGG 10 8 17 8 24 0 100 5 10
    240606163019_giy9 ATTTGCTGGCTCATTCCTAGTCTCATTTTCAG(+AAAAAA)GTACCTTTCTTATAGAGCCCAGGTCCACAGC NGG 10 8 17 8 24 0 100 5 10
    240606163017_2xxr AAAAGGATATACCTTCTTCTCATCACCTCATG(+AAAAAA)GTACCTTCTCCAAAATTGACCATATAATTGG NGG 10 8 17 8 24 0 100 5 10
    240606163016_l7xa TGTGACCAATTATATGGTCAATTTTGGAGAAG(+AAAAAA)GTACCATGAGGTGATGAGAAGAAGGTATATC NGG 10 8 17 8 24 0 100 5 10
    240606163014_5q12 CTGGTCACAACTGGGTACAGCAAACGAGCTGG(+AAAAAA)GTACCCCAAAGATATCTCACCAGTTCTAGTC NGG 10 8 17 8 24 0 100 5 10