Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606163012_ktd2 TCTTCGACTAGAACTGGTGAGATATCTTTGGG(+AAAAAA)GTACCCAGCTCGTTTGCTGTACCCAGTTGTG NGG 10 8 17 8 24 0 100 5 10
    240606163011_yz0v TTAAAAGGCACTTATAATAAAGAGAGCACATG(+AAAAAA)GATCCATCCACTAGACTAAGGCGGGAATGGA NGG 10 8 17 8 24 0 100 5 10
    240606163010_5kaz AATTTTCCATTCCCGCCTTAGTCTAGTGGATG(+AAAAAA)GATCCATGTGCTCTCTTTATTATAAGTGCCT NGG 10 8 17 8 24 0 100 5 10
    240606163008_akmy ATCTTTAAAAAATATTGATGGTATATTGCTTG(+AAAAAA)GTACCAAAAGAAAAGAAAAAATGAAATTCAC NGG 10 8 17 8 24 0 100 5 10
    240606163007_h8f1 CCTGTGTGAATTTCATTTTTTCTTTTCTTTTG(+AAAAAA)GTACCAAGCAATATACCATCAATATTTTTTA NGG 10 8 17 8 24 0 100 5 10
    240606163006_kzl3 TTTGGTTATTCAGATCTTATCCAACACAATTG(+AAAAAA)GATCCATACTAAAAAGAAACCAAACCTTTGT NGG 10 8 17 8 24 0 100 5 10
    240606163004_ew7p TCTTCACAAAGGTTTGGTTTCTTTTTAGTATG(+AAAAAA)GATCCAATTGTGTTGGATAAGATCTGAATAA NGG 10 8 17 8 24 0 100 5 10
    240606163003_9k3d TGGGAGGAACCAACCCATCTCTGTTGAGCAAG(+AAAAAA)GATCCTGGATTGTATAAAAGGAGAAAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240606163001_et9i CTCAGTTCCCTTTCTCCTTTTATACAATCCAG(+AAAAAA)GATCCTTGCTCAACAGAGATGGGTTGGTTCC NGG 10 8 17 8 24 0 100 5 10
    240606162959_44q4 ATGCAGTTCTTACATAAATAACTATTGATAGG(+AAAAAA)GATCCTAAAAGTGATCTCACAGTTGTGTTTA NGG 10 8 17 8 24 0 100 5 10