Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606162632_9w5s GTTCCTCCACAGATGTGCTAAAGTCCAAAGAG(+AAAAAA)GATCCATTTGGAAGGAGTGGCTTGGCCGGGG NGG 10 8 17 8 24 0 100 5 10
    240606162631_ad9s CACGGCCCCGGCCAAGCCACTCCTTCCAAATG(+AAAAAA)GATCCTCTTTGGACTTTAGCACATCTGTGGA NGG 10 8 17 8 24 0 100 5 10
    240606162629_qugu GAGTGGGTAGTTGTGGACTTGTTAACAGTAAG(+AAAAAA)GTACCTTTGAAGTATGCAGATGGAGGTGACC NGG 10 8 17 8 24 0 100 5 10
    240606162628_cobt CACTGGGTCACCTCCATCTGCATACTTCAAAG(+AAAAAA)GTACCTTACTGTTAACAAGTCCACAACTACC NGG 10 8 17 8 24 0 100 5 10
    240606162627_ql82 GACCGAGCTATGCATGGAGGAGGAGGCTGTTG(+AAAAAA)GTACCATACTGAATTTCCACTGGACCTCTAA NGG 10 8 17 8 24 0 100 5 10
    240606162625_wxu7 TGACTTTAGAGGTCCAGTGGAAATTCAGTATG(+AAAAAA)GTACCAACAGCCTCCTCCTCCATGCATAGCT NGG 10 8 17 8 24 0 100 5 10
    240606162624_1dgq GTGGGCATTCATAGTCTAAATCCTCTAGGGAG(+AAAAAA)GATCCTCTTGCCATCTGCCCTCCTCAGTGGC NGG 10 8 17 8 24 0 100 5 10
    240606162623_28oo CCTGGGCCACTGAGGAGGGCAGATGGCAAGAG(+AAAAAA)GATCCTCCCTAGAGGATTTAGACTATGAATG NGG 10 8 17 8 24 0 100 5 10
    240606162621_zeqr GGCAGGCAGATGAGAAACCAGTTGGACCTGTG(+AAAAAA)GATCCCAGTGGTTCCCGTGAGTGGATTTTTA NGG 10 8 17 8 24 0 100 5 10
    240606162619_9ov0 CCCACTAAAAATCCACTCACGGGAACCACTGG(+AAAAAA)GATCCACAGGTCCAACTGGTTTCTCATCTGC NGG 10 8 17 8 24 0 100 5 10