Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606162529_zbhv ATGGACTCCAGGAGTTACACCCTACCAAGAAG(+AAAAAA)GTACCCAGGGGAGCAGTGCAGACACCACACC NGG 10 8 17 8 24 0 100 5 10
    240606162527_i4g3 CTGCTGGTGTGGTGTCTGCACTGCTCCCCTGG(+AAAAAA)GTACCTTCTTGGTAGGGTGTAACTCCTGGAG NGG 10 8 17 8 24 0 100 5 10
    240606162525_d4iv AAACATTCACAGTTTCACTTCCCTTGTGTGTG(+AAAAAA)GTACCTAGACAGGGCATCTTCTTCAGATAGT NGG 10 8 17 8 24 0 100 5 10
    240606162524_2fwn CCAGTACTATCTGAAGAAGATGCCCTGTCTAG(+AAAAAA)GTACCACACACAAGGGAAGTGAAACTGTGAA NGG 10 8 17 8 24 0 100 5 10
    240606162522_2mbp AAGCCTAATAAATGTGCAAAGGTGGGGGGAAG(+AAAAAA)GATCCCACTTCCCATTTTCTATTTCACCTGT NGG 10 8 17 8 24 0 100 5 10
    240606162521_epkc CACCAACAGGTGAAATAGAAAATGGGAAGTGG(+AAAAAA)GATCCTTCCCCCCACCTTTGCACATTTATTA NGG 10 8 17 8 24 0 100 5 10
    240606162519_4kk6 AACCAGACATGATACCCCAGGTTTTATTTCTG(+AAAAAA)GATCCCACATGGTGGAAGGGGAGAAGTGACC NGG 10 8 17 8 24 0 100 5 10
    240606162518_ud4f TGTTGGGTCACTTCTCCCCTTCCACCATGTGG(+AAAAAA)GATCCAGAAATAAAACCTGGGGTATCATGTC NGG 10 8 17 8 24 0 100 5 10
    240606162516_fojy CTTTTGGCAATCCTATCGGAAAGAATTTTAGG(+AAAAAA)GATCCAGGAGAGCTACTAATACGAGATACCA NGG 10 8 17 8 24 0 100 5 10
    240606162514_qa5l GCAAATGGTATCTCGTATTAGTAGCTCTCCTG(+AAAAAA)GATCCCTAAAATTCTTTCCGATAGGATTGCC NGG 10 8 17 8 24 0 100 5 10