Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606162512_k0ut TAGCAACAAGAATCCAAGTGTACACTGTGGTG(+AAAAAA)GATCCAGCTTCAAAGCAAACTGTTGCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240606162511_2bxx GTGTTTGGAGGCAACAGTTTGCTTTGAAGCTG(+AAAAAA)GATCCACCACAGTGTACACTTGGATTCTTGT NGG 10 8 17 8 24 0 100 5 10
    240606162510_rdw4 GTGAAATCAAGATTAGTTAAACCATCTAAACG(+AAAAAA)GATCCATAACCCCTAAGGAAATACAAAGAGT NGG 10 8 17 8 24 0 100 5 10
    240606162508_khrg TAATGACTCTTTGTATTTCCTTAGGGGTTATG(+AAAAAA)GATCCGTTTAGATGGTTTAACTAATCTTGAT NGG 10 8 17 8 24 0 100 5 10
    240606162506_0zg5 GGAAAGACCATCCAGAGACTGTCCTACCAGGG(+AAAAAA)GATCCATCCCATATACAACCACCAAACACAG NGG 10 8 17 8 24 0 100 5 10
    240606162504_enuf AGTGTCTGTGTTTGGTGGTTGTATATGGGATG(+AAAAAA)GATCCCCTGGTAGGACAGTCTCTGGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606162503_n892 CAAAGGCAGCTGCAGAGCTCAGCCTTGGGTAG(+AAAAAA)GATCCTGTCTGAGGACACTGACTGAGGACAT NGG 10 8 17 8 24 0 100 5 10
    240606162502_yu7o CAGGGATGTCCTCAGTCAGTGTCCTCAGACAG(+AAAAAA)GATCCTACCCAAGGCTGAGCTCTGCAGCTGC NGG 10 8 17 8 24 0 100 5 10
    240606162500_8c5j CTGCCATGGTCCTAGAAAATTTGCATCACCAG(+AAAAAA)GATCCTGGAGGGACAAGGGTGGAAAGTATTT NGG 10 8 17 8 24 0 100 5 10
    240606162458_tn1o ATTTGAAATACTTTCCACCCTTGTCCCTCCAG(+AAAAAA)GATCCTGGTGATGCAAATTTTCTAGGACCAT NGG 10 8 17 8 24 0 100 5 10