Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606162427_n034 TGGACACCTCACAGATGCAGAGGCCACTCTTG(+AAAAAA)GATCCAGCATGTACTGTAGAACACTTCCCCA NGG 10 8 17 8 24 0 100 5 10
    240606162425_m0w8 ATAACTGGGGAAGTGTTCTACAGTACATGCTG(+AAAAAA)GATCCAAGAGTGGCCTCTGCATCTGTGAGGT NGG 10 8 17 8 24 0 100 5 10
    240606162424_ffl4 GCAGGGGTCTGGTCAGCTGGGCTGCAGGTCCG(+AAAAAA)GTACCCTTAAAGTCTCTGAAAGGATGAAAAG NGG 10 8 17 8 24 0 100 5 10
    240606162422_bw2x CCTCTCTTTTCATCCTTTCAGAGACTTTAAGG(+AAAAAA)GTACCGGACCTGCAGCCCAGCTGACCAGACC NGG 10 8 17 8 24 0 100 5 10
    240606162421_5wuu CATTAACTAGATATAATGATTTTGCCTTTTTG(+AAAAAA)GTACCTAGTGAAATCTAGAGTATTTTACATT NGG 10 8 17 8 24 0 100 5 10
    240606162419_4ud5 GCTGTAATGTAAAATACTCTAGATTTCACTAG(+AAAAAA)GTACCAAAAAGGCAAAATCATTATATCTAGT NGG 10 8 17 8 24 0 100 5 10
    240606162418_p550 TACCCAGTTCTATTTCTCCTGATAAGATGAAG(+AAAAAA)GTACCTTGAAATCCTGGGAAGGGCTGACATC NGG 10 8 17 8 24 0 100 5 10
    240606162417_i2ns AAGATGATGTCAGCCCTTCCCAGGATTTCAAG(+AAAAAA)GTACCTTCATCTTATCAGGAGAAATAGAACT NGG 10 8 17 8 24 0 100 5 10
    240606162416_kl6p TTGCTAGAATGGTTCCTAGAACTCAGGAAAAG(+AAAAAA)GTACCTTATAAAACCATCACTGGTTTATTTT NGG 10 8 17 8 24 0 100 5 10
    240606162414_lubt CTTTTAAAATAAACCAGTGATGGTTTTATAAG(+AAAAAA)GTACCTTTTCCTGAGTTCTAGGAACCATTCT NGG 10 8 17 8 24 0 100 5 10