Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606162341_3cqo AAAAATCCCCATACAAAAGTGAAGTCCCATTG(+AAAAAA)GTACCCCTGCCATCATCTCCTAGTGGCTATT NGG 10 8 17 8 24 0 100 5 10
    240606162339_yrdd CACAGAATAGCCACTAGGAGATGATGGCAGGG(+AAAAAA)GTACCAATGGGACTTCACTTTTGTATGGGGA NGG 10 8 17 8 24 0 100 5 10
    240606162338_inoy TATAATCCTGAACATGAAGTGTTTCTAAAAGG(+AAAAAA)GATCCTCCTTCCCAGTAGACAGAAAAAAGTC NGG 10 8 17 8 24 0 100 5 10
    240606162336_wq75 GGCTCGACTTTTTTCTGTCTACTGGGAAGGAG(+AAAAAA)GATCCCTTTTAGAAACACTTCATGTTCAGGA NGG 10 8 17 8 24 0 100 5 10
    240606162335_d8mz TTTAGTCTTCTGCAACAACAGCCACCAAAGTG(+AAAAAA)GATCCTAGGCGTTCCTGGCAGTCACCACTGG NGG 10 8 17 8 24 0 100 5 10
    240606162333_5sej GCTTGCCAGTGGTGACTGCCAGGAACGCCTAG(+AAAAAA)GATCCACTTTGGTGGCTGTTGTTGCAGAAGA NGG 10 8 17 8 24 0 100 5 10
    240606162330_2cm0 TGGACTTTCATAGGACATAAGCTTGAACCCCG(+AAAAAA)GTACCGCTCAGCAGAACAGTTGGGTTCTCAG NGG 10 8 17 8 24 0 100 5 10
    240606162329_d5i1 AGGCACTGAGAACCCAACTGTTCTGCTGAGCG(+AAAAAA)GTACCGGGGTTCAAGCTTATGTCCTATGAAA NGG 10 8 17 8 24 0 100 5 10
    240606162328_9mvz AGAACCTGCCACAGCTTAACACACCTTATCTG(+AAAAAA)GTACCTGTCTGAAAGCACTCCCTGGGCCTAT NGG 10 8 17 8 24 0 100 5 10
    240606162327_4zvo CATAGATAGGCCCAGGGAGTGCTTTCAGACAG(+AAAAAA)GTACCAGATAAGGTGTGTTAAGCTGTGGCAG NGG 10 8 17 8 24 0 100 5 10