Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606163521_kz0t GAGCTCCATTAAAGTCTCATGACATGCAAGTG(+AAAAAA)GATCCTCAGAGCAGATCCCCGTGAGGCATAG NGG 10 8 17 8 24 0 100 5 10
    240606163520_cptp TCCCTCTATGCCTCACGGGGATCTGCTCTGAG(+AAAAAA)GATCCACTTGCATGTCATGAGACTTTAATGG NGG 10 8 17 8 24 0 100 5 10
    240606163518_38ia CCATAAACCTGGAATATGTGTTTATCAGTAGG(+AAAAAA)GTACCCTACTTGAGCAGGAACCCTGAATAAA NGG 10 8 17 8 24 0 100 5 10
    240606163516_h5dg ACCAATTTATTCAGGGTTCCTGCTCAAGTAGG(+AAAAAA)GTACCCTACTGATAAACACATATTCCAGGTT NGG 10 8 17 8 24 0 100 5 10
    240606163514_yzbo AGAACTTGTAGCACCTTTAACAGATTCCAAGG(+AAAAAA)GATCCAGTGTCTTCTCTTGGTTTCTTCAGGC NGG 10 8 17 8 24 0 100 5 10
    240606163513_6257 GAAATGCCTGAAGAAACCAAGAGAAGACACTG(+AAAAAA)GATCCCTTGGAATCTGTTAAAGGTGCTACAA NGG 10 8 17 8 24 0 100 5 10
    240606163511_jzio CCTAGCATTGCTGATTGTTTCACACAAGGTAG(+AAAAAA)GATCCGTTAGCAATTAAGTGCAACGACTCAT NGG 10 8 17 8 24 0 100 5 10
    240606163510_vl0b CCAAAATGAGTCGTTGCACTTAATTGCTAACG(+AAAAAA)GATCCTACCTTGTGTGAAACAATCAGCAATG NGG 10 8 17 8 24 0 100 5 10
    240606163509_6xw7 AAAACACTAAGAATGCATTTTAGCCAGGTATG(+AAAAAA)GTACCAGGAAAGAGATGGCACATAAAGGCAC NGG 10 8 17 8 24 0 100 5 10
    240606163507_6lrm TCTGGGTGCCTTTATGTGCCATCTCTTTCCTG(+AAAAAA)GTACCATACCTGGCTAAAATGCATTCTTAGT NGG 10 8 17 8 24 0 100 5 10