Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606162311_yzkm TGGTCTGGACAGAGGTGTGCCCCATCCAGACG(+AAAAAA)GTACCAGGATAGCAGCAGCTGCATGGCGCTA NGG 10 8 17 8 24 0 100 5 10
    240606162309_ay32 ACAGGTAGCGCCATGCAGCTGCTGCTATCCTG(+AAAAAA)GTACCGTCTGGATGGGGCACACCTCTGTCCA NGG 10 8 17 8 24 0 100 5 10
    240606162306_tgex CTGCCCACTGGCCGTGCGCAGGGAGAACCTGG(+AAAAAA)GATCCCATTCATCGGAGTGACACATCACGAT NGG 10 8 17 8 24 0 100 5 10
    240606162305_u97u TGAGCATCGTGATGTGTCACTCCGATGAATGG(+AAAAAA)GATCCCAGGTTCTCCCTGCGCACGGCCAGTG NGG 10 8 17 8 24 0 100 5 10
    240606162303_uv37 ACAGAAGGGGAAGTCATGTTCACATAACGCGG(+AAAAAA)GTACCTCACCAAACACACTTTTGTCTGAGTA NGG 10 8 17 8 24 0 100 5 10
    240606162302_rt05 GGTGATACTCAGACAAAAGTGTGTTTGGTGAG(+AAAAAA)GTACCCGCGTTATGTGAACATGACTTCCCCT NGG 10 8 17 8 24 0 100 5 10
    240606162300_adqy GCCATGCACAGTGGTACACATCTGCAATCCTG(+AAAAAA)GTACCTGACAAGCTGAGACAGAAGGATCACA NGG 10 8 17 8 24 0 100 5 10
    240606162259_t4n7 GAACTTGTGATCCTTCTGTCTCAGCTTGTCAG(+AAAAAA)GTACCAGGATTGCAGATGTGTACCACTGTGC NGG 10 8 17 8 24 0 100 5 10
    240606162257_1466 GCCACCATACAGGATCACCTACAGAAAACAGG(+AAAAAA)GATCCTCAAGGGAGGACAAATCCCCCCACAG NGG 10 8 17 8 24 0 100 5 10
    240606162256_imvm ACGTTCTGTGGGGGGATTTGTCCTCCCTTGAG(+AAAAAA)GATCCCTGTTTTCTGTAGGTGATCCTGTATG NGG 10 8 17 8 24 0 100 5 10