Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606162240_xyng TAATAAGTCCGTTAAAAGCAGAGCCAGGACTG(+AAAAAA)GATCCCGAAAGAATAGGCTTTTGAAACAATC NGG 10 8 17 8 24 0 100 5 10
    240606162239_169h CATGTGATTGTTTCAAAAGCCTATTCTTTCGG(+AAAAAA)GATCCAGTCCTGGCTCTGCTTTTAACGGACT NGG 10 8 17 8 24 0 100 5 10
    240606162238_yi10 TCACATCATTTTCTTCCAGTTATTGGCTACAG(+AAAAAA)GATCCAAGGGGAAGGTTTTCTGCTTCCAGCT NGG 10 8 17 8 24 0 100 5 10
    240606162235_03bq CTGTGAGCTGGAAGCAGAAAACCTTCCCCTTG(+AAAAAA)GATCCTGTAGCCAATAACTGGAAGAAAATGA NGG 10 8 17 8 24 0 100 5 10
    240606162234_ibiq ATGTGCCAAGGCTCCAACAGACAAGATTATTG(+AAAAAA)GTACCTCTTTGTAGGGCAGATTTATAGTTAT NGG 10 8 17 8 24 0 100 5 10
    240606162232_o7tk GAATTATAACTATAAATCTGCCCTACAAAGAG(+AAAAAA)GTACCAATAATCTTGTCTGTTGGAGCCTTGG NGG 10 8 17 8 24 0 100 5 10
    240606162231_7eq4 TCCACGCTCTGTCAATGCGTTGCTCAGCTAAG(+AAAAAA)GATCCCCGGGGAGCCCTTGCTAGCTGGTCAA NGG 10 8 17 8 24 0 100 5 10
    240606162228_x82b AGAGGTTGACCAGCTAGCAAGGGCTCCCCGGG(+AAAAAA)GATCCTTAGCTGAGCAACGCATTGACAGAGC NGG 10 8 17 8 24 0 100 5 10
    240606162225_l37d CATCTTGGAATATTTCCCCAGAGGATAAGGGG(+AAAAAA)GATCCACTGTGTCTTGTTTTGGCCTGGGATC NGG 10 8 17 8 24 0 100 5 10
    240606162224_3drj GGAGTGATCCCAGGCCAAAACAAGACACAGTG(+AAAAAA)GATCCCCCTTATCCTCTGGGGAAATATTCCA NGG 10 8 17 8 24 0 100 5 10