Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606162136_9zmw GTTACTTTAAGTCCTGAGATGCAGGTTCCCTG(+AAAAAA)GTACCATTGAGTTGCTGCTACTAAAAGCTCA NGG 10 8 17 8 24 0 100 5 10
    240606162135_0rxs GCATGTGAGCTTTTAGTAGCAGCAACTCAATG(+AAAAAA)GTACCAGGGAACCTGCATCTCAGGACTTAAA NGG 10 8 17 8 24 0 100 5 10
    240606162134_wic6 AGATACAGCTGTCCCGTGGAGCAGCAGGCATG(+AAAAAA)GATCCCCTCCATCCTTGGGCTTCCCGGGCCC NGG 10 8 17 8 24 0 100 5 10
    240606162132_1652 CGCAGGGGCCCGGGAAGCCCAAGGATGGAGGG(+AAAAAA)GATCCATGCCTGCTGCTCCACGGGACAGCTG NGG 10 8 17 8 24 0 100 5 10
    240606162130_i9lj GCCTATTTTGTGATGTGATGACCTTCAAATTG(+AAAAAA)GATCCCGTGGCTTGTCCTCTCTGAAGGCCGG NGG 10 8 17 8 24 0 100 5 10
    240606162128_lbgh GGGCTCCGGCCTTCAGAGAGGACAAGCCACGG(+AAAAAA)GATCCAATTTGAAGGTCATCACATCACAAAA NGG 10 8 17 8 24 0 100 5 10
    240606162126_2wxb AGGACCCACTGACTAGCAAATAGTAGGGTCCG(+AAAAAA)GATCCGGTTCTAATCTGGGGAAGGGGCTCAG NGG 10 8 17 8 24 0 100 5 10
    240606162125_o7vh ACAAACTGAGCCCCTTCCCCAGATTAGAACCG(+AAAAAA)GATCCGGACCCTACTATTTGCTAGTCAGTGG NGG 10 8 17 8 24 0 100 5 10
    240606162123_rdfy ATCTAGTCATTCCAGACAGCTTTCTGTATGTG(+AAAAAA)GATCCTGGCTTTGGATCAAAACCACTTCTGC NGG 10 8 17 8 24 0 100 5 10
    240606162121_k3rg TTATTGCAGAAGTGGTTTTGATCCAAAGCCAG(+AAAAAA)GATCCACATACAGAAAGCTGTCTGGAATGAC NGG 10 8 17 8 24 0 100 5 10