Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606163325_wz3l GCGCCAGTCCTCCGATAGACTGCGTCGCCCGG(+AAAAAA)GTACCCGTGTTCCCAATAAAGCCTCTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240606163324_ce8p GCAAACAGCAAGAGGCTTTATTGGGAACACGG(+AAAAAA)GTACCCGGGCGACGCAGTCTATCGGAGGACT NGG 10 8 17 8 24 0 100 5 10
    240606163323_yr08 TAGAAGCAGGTTCTTGGAAGTTACAATTTAAG(+AAAAAA)GTACCCCCAAAAAGTTGGAAATAAAAGGAAA NGG 10 8 17 8 24 0 100 5 10
    240606163321_wd99 ATTTGTTTCCTTTTATTTCCAACTTTTTGGGG(+AAAAAA)GTACCTTAAATTGTAACTTCCAAGAACCTGC NGG 10 8 17 8 24 0 100 5 10
    240606163320_o4bh ACATACACAGCTACAGAGTGAGAGTTGGTTTG(+AAAAAA)GATCCTCTTCATTTCATTTGTTTGCCTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606163318_ypbl ATAACAGAAAGGCAAACAAATGAAATGAAGAG(+AAAAAA)GATCCAAACCAACTCTCACTCTGTAGCTGTG NGG 10 8 17 8 24 0 100 5 10
    240606163316_b5gu CTCTTGTGTGTCCTGCCATCAGTGTGACCAGG(+AAAAAA)GTACCTCAATAAAGAGTGGTCGAGGAAAGGT NGG 10 8 17 8 24 0 100 5 10
    240606163315_v4u3 GAGAAACCTTTCCTCGACCACTCTTTATTGAG(+AAAAAA)GTACCCTGGTCACACTGATGGCAGGACACAC NGG 10 8 17 8 24 0 100 5 10
    240606163314_iy9m ACCTAACTGTGCAGGCTGACGATTAATTACTG(+AAAAAA)GATCCAAGAGAACAATACATTCTTATGTACA NGG 10 8 17 8 24 0 100 5 10
    240606163312_a8p2 TCTTGTGTACATAAGAATGTATTGTTCTCTTG(+AAAAAA)GATCCAGTAATTAATCGTCAGCCTGCACAGT NGG 10 8 17 8 24 0 100 5 10