Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606162207_bxar CAGACCCTTTTACATCACAAATGGGAAGACTG(+AAAAAA)GTACCCCAAAGGCTGGCTGGAATGCTTAAGG NGG 10 8 17 8 24 0 100 5 10
    240606162204_4lz1 AGTGGCCTTAAGCATTCCAGCCAGCCTTTGGG(+AAAAAA)GTACCAGTCTTCCCATTTGTGATGTAAAAGG NGG 10 8 17 8 24 0 100 5 10
    240606162203_ba5y TGCTGATGACTAACTTTTGCAGATAAGCATAG(+AAAAAA)GTACCTGTGTTCAACAGATGCCAGCAGAAGT NGG 10 8 17 8 24 0 100 5 10
    240606162202_hagq AAGATACTTCTGCTGGCATCTGTTGAACACAG(+AAAAAA)GTACCTATGCTTATCTGCAAAAGTTAGTCAT NGG 10 8 17 8 24 0 100 5 10
    240606162200_srq2 ACACACACCCCAGGTCTGTACCAGGACTTTGG(+AAAAAA)GATCCCAAGCTGTTTTCAAGCTGTTTCTGAG NGG 10 8 17 8 24 0 100 5 10
    240606162158_vp0z TGAGCCTCAGAAACAGCTTGAAAACAGCTTGG(+AAAAAA)GATCCCAAAGTCCTGGTACAGACCTGGGGTG NGG 10 8 17 8 24 0 100 5 10
    240606162157_xkvo CTTCTTTGTTTACTGTATGAGATTGACACCTG(+AAAAAA)GATCCATTATTTGCTGCTATAAATATGGCTA NGG 10 8 17 8 24 0 100 5 10
    240606162155_h90w CTCTGTAGCCATATTTATAGCAGCAAATAATG(+AAAAAA)GATCCAGGTGTCAATCTCATACAGTAAACAA NGG 10 8 17 8 24 0 100 5 10
    240606162154_m35g CCAAAAGTAATAAAGCACTAATAAAATTAATG(+AAAAAA)GATCCAAAGTAACAATTACTGTCTATGTTCT NGG 10 8 17 8 24 0 100 5 10
    240606162153_nu22 ACAAAAGAACATAGACAGTAATTGTTACTTTG(+AAAAAA)GATCCATTAATTTTATTAGTGCTTTATTACT NGG 10 8 17 8 24 0 100 5 10