Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606162151_dqln TCTTTCTAAGGCAAGATCAGCCATCACATGTG(+AAAAAA)GTACCTTCCATGCATTCTGAACTCTCTCTCT NGG 10 8 17 8 24 0 100 5 10
    240606162150_685t GAGAGAGAGAGAGAGTTCAGAATGCATGGAAG(+AAAAAA)GTACCACATGTGATGGCTGATCTTGCCTTAG NGG 10 8 17 8 24 0 100 5 10
    240606162149_geqd TCATGCCCTGCCCCAAAGTAAAGGGTAAGTAG(+AAAAAA)GTACCATTTAGTGAAGGCCAGTATCATTTCA NGG 10 8 17 8 24 0 100 5 10
    240606162147_n2a9 AACAGTGAAATGATACTGGCCTTCACTAAATG(+AAAAAA)GTACCTACTTACCCTTTACTTTGGGGCAGGG NGG 10 8 17 8 24 0 100 5 10
    240606162146_rxg9 AGTTAAGCTTGCACATTTGGATAGGAAGGTGG(+AAAAAA)GTACCCCGCTCCAGCAAGATCGTTTTATGCA NGG 10 8 17 8 24 0 100 5 10
    240606162144_k43u GCTTGTGCATAAAACGATCTTGCTGGAGCGGG(+AAAAAA)GTACCCACCTTCCTATCCAAATGTGCAAGCT NGG 10 8 17 8 24 0 100 5 10
    240606162142_cp8x AGGTAGAGCCCTGAGAGTTCCCAGCAGAGTGG(+AAAAAA)GATCCAGACTTTGCACAGAAATGATCTCAGG NGG 10 8 17 8 24 0 100 5 10
    240606162141_hxyj GGTGACCTGAGATCATTTCTGTGCAAAGTCTG(+AAAAAA)GATCCCACTCTGCTGGGAACTCTCAGGGCTC NGG 10 8 17 8 24 0 100 5 10
    240606162140_beye CCGGCCTCCTCCCCTCACACACTCTGGACTTG(+AAAAAA)GTACCGAATAATCCCGTGTGGTCCTAGCTGA NGG 10 8 17 8 24 0 100 5 10
    240606162138_ume1 GTGCTTCAGCTAGGACCACACGGGATTATTCG(+AAAAAA)GTACCAAGTCCAGAGTGTGTGAGGGGAGGAG NGG 10 8 17 8 24 0 100 5 10