Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606162120_0q2n CACGACTCTCCCAGGACTGCAGTCCCATGAAG(+AAAAAA)GATCCTCTTTCTTTCTATAAGGATGCTGACC NGG 10 8 17 8 24 0 100 5 10
    240606162119_79cv CCTGAGGTCAGCATCCTTATAGAAAGAAAGAG(+AAAAAA)GATCCTTCATGGGACTGCAGTCCTGGGAGAG NGG 10 8 17 8 24 0 100 5 10
    240606162117_03ba AATTTCATTAAAAAGCTTAGCCTATCTGTCTG(+AAAAAA)GTACCCTCAAAGCTGATGCCTCTGGATCAGA NGG 10 8 17 8 24 0 100 5 10
    240606162115_3zyz AAGGTTCTGATCCAGAGGCATCAGCTTTGAGG(+AAAAAA)GTACCAGACAGATAGGCTAAGCTTTTTAATG NGG 10 8 17 8 24 0 100 5 10
    240606162114_w55v GAGAAAGGTTGACTCACTCAGCTATCCTGCAG(+AAAAAA)GTACCTTCTGTTCTACCTAAGACAGAACAAT NGG 10 8 17 8 24 0 100 5 10
    240606162113_c3tv TTGTCATTGTTCTGTCTTAGGTAGAACAGAAG(+AAAAAA)GTACCTGCAGGATAGCTGAGTGAGTCAACCT NGG 10 8 17 8 24 0 100 5 10
    240606162111_k6hc AGAATTTACCTTTACAATTTATTTTCAAACTG(+AAAAAA)GATCCATTCAATTGTCATGTATGTGTGCCTA NGG 10 8 17 8 24 0 100 5 10
    240606162110_d2wr CACAGTAGGCACACATACATGACAATTGAATG(+AAAAAA)GATCCAGTTTGAAAATAAATTGTAAAGGTAA NGG 10 8 17 8 24 0 100 5 10
    240606162109_ef55 TTTTCAAGTCAAAATTTTAGGACAAATTTTAG(+AAAAAA)GATCCTGGTTATCATATTATAGTATTCAGGA NGG 10 8 17 8 24 0 100 5 10
    240606162107_ffeq CAAAATCCTGAATACTATAATATGATAACCAG(+AAAAAA)GATCCTAAAATTTGTCCTAAAATTTTGACTT NGG 10 8 17 8 24 0 100 5 10