Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606162021_b1n1 TAAAAACCCTCATCAAACTACTTCATTTCCAG(+AAAAAA)GATCCCCACATTTCCACTTTTGTGAAGCTGT NGG 10 8 17 8 24 0 100 5 10
    240606162019_dhb5 TTTTAACAGCTTCACAAAAGTGGAAATGTGGG(+AAAAAA)GATCCTGGAAATGAAGTAGTTTGATGAGGGT NGG 10 8 17 8 24 0 100 5 10
    240606162018_oi9h AGATGACTCTCCATGAGAAGACTTGAAAACTG(+AAAAAA)GTACCAGGAGTGGGCTGAAGATTCCCATGAC NGG 10 8 17 8 24 0 100 5 10
    240606162016_y9sd ATGACGTCATGGGAATCTTCAGCCCACTCCTG(+AAAAAA)GTACCAGTTTTCAAGTCTTCTCATGGAGAGT NGG 10 8 17 8 24 0 100 5 10
    240606162015_ek66 AGTCCCTCAAACTATACTGATGATCAAATAAG(+AAAAAA)GATCCGAACCACTACTAGAAATGCCCAACTA NGG 10 8 17 8 24 0 100 5 10
    240606162014_ushs AGATCTAGTTGGGCATTTCTAGTAGTGGTTCG(+AAAAAA)GATCCTTATTTGATCATCAGTATAGTTTGAG NGG 10 8 17 8 24 0 100 5 10
    240606162012_ukbg ACAGCTCCTGTGTCCTTGTCTCTAGCAGGGCG(+AAAAAA)GTACCTGTCAGCAGCCAAGGGCAAGGCTTGC NGG 10 8 17 8 24 0 100 5 10
    240606162010_tbds TTCCAGCAAGCCTTGCCCTTGGCTGCTGACAG(+AAAAAA)GTACCGCCCTGCTAGAGACAAGGACACAGGA NGG 10 8 17 8 24 0 100 5 10
    240606162009_g9z4 GCTTAAGTATAGATAACCCTATATCAGGGGAG(+AAAAAA)GTACCGTGTTCTAATGCTGCAAGTGCTTGGG NGG 10 8 17 8 24 0 100 5 10
    240606162007_rrhw GATAGCCCAAGCACTTGCAGCATTAGAACACG(+AAAAAA)GTACCTCCCCTGATATAGGGTTATCTATACT NGG 10 8 17 8 24 0 100 5 10