Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606161929_8rkm GCACAGCTGCCAAGGCTTAGAAGGAGAAGCTG(+AAAAAA)GTACCGGCCTCTTCTTCAAAAGCAGCCAGGG NGG 10 8 17 8 24 0 100 5 10
    240606161927_kgo7 CAGCACCCTGGCTGCTTTTGAAGAAGAGGCCG(+AAAAAA)GTACCAGCTTCTCCTTCTAAGCCTTGGCAGC NGG 10 8 17 8 24 0 100 5 10
    240606161925_ak4j GACATTTGACAAGTGTCCAATTTCTCCAAAGG(+AAAAAA)GTACCTTAAGATTCTCAAAAAAAAAGTAAAT NGG 10 8 17 8 24 0 100 5 10
    240606161924_5m75 TTTGAATTTACTTTTTTTTTGAGAATCTTAAG(+AAAAAA)GTACCCTTTGGAGAAATTGGACACTTGTCAA NGG 10 8 17 8 24 0 100 5 10
    240606161922_wcuu ATTAATGTGGTTAAATCCAAAGTCTGAGGTAG(+AAAAAA)GTACCTGAGCCTTGTTATTTTGGAAATTCAA NGG 10 8 17 8 24 0 100 5 10
    240606161921_x0b3 TGAATTTGAATTTCCAAAATAACAAGGCTCAG(+AAAAAA)GTACCTACCTCAGACTTTGGATTTAACCACA NGG 10 8 17 8 24 0 100 5 10
    240606161920_nr4u TGAGTGCTTGAGGAGGCCAGAAGAGGGTGTTG(+AAAAAA)GATCCCCTGGAGCTGAAGTAATGGGCCATTG NGG 10 8 17 8 24 0 100 5 10
    240606161917_6bg6 GCTTACAATGGCCCATTACTTCAGCTCCAGGG(+AAAAAA)GATCCAACACCCTCTTCTGGCCTCCTCAAGC NGG 10 8 17 8 24 0 100 5 10
    240606161916_31up AGTCCCAATTTCAGGGTGGCACAAATTGTATG(+AAAAAA)GATCCAGCTCTTGGATCACACAATCGCAAAG NGG 10 8 17 8 24 0 100 5 10
    240606161914_bi81 GTAGCCTTTGCGATTGTGTGATCCAAGAGCTG(+AAAAAA)GATCCATACAATTTGTGCCACCCTGAAATTG NGG 10 8 17 8 24 0 100 5 10