Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606162036_347x CAAGTTGCTATGCAAGTGGAAGGACTGTTTTG(+AAAAAA)GATCCCCAGCACCCATGTAAAGTCAAATGAG NGG 10 8 17 8 24 0 100 5 10
    240606162034_ku5y ACATACTCATTTGACTTTACATGGGTGCTGGG(+AAAAAA)GATCCAAAACAGTCCTTCCACTTGCATAGCA NGG 10 8 17 8 24 0 100 5 10
    240606162033_u0q5 AACCAAAAACAAAAAAACAAAACAGAGTAGAG(+AAAAAA)GTACCATTTCATGAAAGAAAAATGAGAAGGG NGG 10 8 17 8 24 0 100 5 10
    240606162031_87vk ACACACCCTTCTCATTTTTCTTTCATGAAATG(+AAAAAA)GTACCTCTACTCTGTTTTGTTTTTTTGTTTT NGG 10 8 17 8 24 0 100 5 10
    240606162030_lfs4 TACACTGTGCCAGCTCTTTGTCACTTGACTTG(+AAAAAA)GTACCTGAAACAAACAAACAAAAAAATTAAA NGG 10 8 17 8 24 0 100 5 10
    240606162029_mg9x ACCATTTTAATTTTTTTGTTTGTTTGTTTCAG(+AAAAAA)GTACCAAGTCAAGTGACAAAGAGCTGGCACA NGG 10 8 17 8 24 0 100 5 10
    240606162028_u5mf CACAGTATGCACACAGACAACTGTGAATTCAG(+AAAAAA)GTACCCTTCCACAAGCCTTCCCTCTTGTACT NGG 10 8 17 8 24 0 100 5 10
    240606162026_arxt TGGAAAGTACAAGAGGGAAGGCTTGTGGAAGG(+AAAAAA)GTACCTGAATTCACAGTTGTCTGTGTGCATA NGG 10 8 17 8 24 0 100 5 10
    240606162024_4tjx TGCTATCCTGGGTAAGAACGCCCTGAGTACGG(+AAAAAA)GTACCATGCTCTGTTTACTTTAGGATCTCCA NGG 10 8 17 8 24 0 100 5 10
    240606162023_98x3 GGTGCTGGAGATCCTAAAGTAAACAGAGCATG(+AAAAAA)GTACCCGTACTCAGGGCGTTCTTACCCAGGA NGG 10 8 17 8 24 0 100 5 10