Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606161846_lnck AACAGAAACTTTTATATTGTGCTGCTGCCTTG(+AAAAAA)GTACCACCCTTCAGTCTGTTCAAGAAACACC NGG 10 8 17 8 24 0 100 5 10
    240606161844_ztuy GTCTTGGTGTTTCTTGAACAGACTGAAGGGTG(+AAAAAA)GTACCAAGGCAGCAGCACAATATAAAAGTTT NGG 10 8 17 8 24 0 100 5 10
    240606161841_83nj TGGTCAAATCACAGGTGCTTATGCAGCCCTGG(+AAAAAA)GATCCTTCCCGTGTGAACCCTGTGGAGCACA NGG 10 8 17 8 24 0 100 5 10
    240606161839_ud4o GCACATGTGCTCCACAGGGTTCACACGGGAAG(+AAAAAA)GATCCCAGGGCTGCATAAGCACCTGTGATTT NGG 10 8 17 8 24 0 100 5 10
    240606161838_80zy ACTGTCTCCAGACACACCAGAAGAGGGCATCG(+AAAAAA)GATCCCATTACAGAATGTTGTGAGCCACTAT NGG 10 8 17 8 24 0 100 5 10
    240606161836_cuzb GCCACATAGTGGCTCACAACATTCTGTAATGG(+AAAAAA)GATCCGATGCCCTCTTCTGGTGTGTCTGGAG NGG 10 8 17 8 24 0 100 5 10
    240606161835_88gn CTCCAAACCCAACTTTTTACATAGGTGCTGAG(+AAAAAA)GATCCAAACTCAGGTGTTTGTGCCTGCATAT NGG 10 8 17 8 24 0 100 5 10
    240606161834_llev GCTTGATATGCAGGCACAAACACCTGAGTTTG(+AAAAAA)GATCCTCAGCACCTATGTAAAAAGTTGGGTT NGG 10 8 17 8 24 0 100 5 10
    240606161833_r6ah CCACGCCTCCCCACCCCCACCTCCCAGCACTG(+AAAAAA)GTACCTTTACAGTGATACTGAGCTGCGTACT NGG 10 8 17 8 24 0 100 5 10
    240606161832_uczn CTCTGAGTACGCAGCTCAGTATCACTGTAAAG(+AAAAAA)GTACCAGTGCTGGGAGGTGGGGGTGGGGAGG NGG 10 8 17 8 24 0 100 5 10