Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606161737_0uv0 CAGCTCCCTTCAGTGAGGTCTGGACCCCACTG(+AAAAAA)GATCCATCATCTGTGCGATCTTGGCTGTCAG NGG 10 8 17 8 24 0 100 5 10
    240606161736_r7ss AAGATCTGACAGCCAAGATCGCACAGATGATG(+AAAAAA)GATCCAGTGGGGTCCAGACCTCACTGAAGGG NGG 10 8 17 8 24 0 100 5 10
    240606161734_6187 ACGTGTCTTTCCTTTGCTTCCATGAAAGCAGG(+AAAAAA)GATCCAGTTAGTGTAATGCACTGTTTGGCTT NGG 10 8 17 8 24 0 100 5 10
    240606161733_s71r AAAATAAGCCAAACAGTGCATTACACTAACTG(+AAAAAA)GATCCCTGCTTTCATGGAAGCAAAGGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240606161732_9fsv TTCTGACCCTCCTAAAGAGTGATGTACACACG(+AAAAAA)GTACCCCCCACATGCAGTGCTATCTGTAAAC NGG 10 8 17 8 24 0 100 5 10
    240606161730_jak8 GTACGGTTTACAGATAGCACTGCATGTGGGGG(+AAAAAA)GTACCGTGTGTACATCACTCTTTAGGAGGGT NGG 10 8 17 8 24 0 100 5 10
    240606161728_55eq GACTGGCAGGTATTGTAGGTGTTGTGACTGTG(+AAAAAA)GTACCTCCTAGGAATGCTGTTGGAGACAGAA NGG 10 8 17 8 24 0 100 5 10
    240606161727_u7x5 TGGGATTCTGTCTCCAACAGCATTCCTAGGAG(+AAAAAA)GTACCACAGTCACAACACCTACAATACCTGC NGG 10 8 17 8 24 0 100 5 10
    240606161726_gn1b CAAGCTGGACTGAGCCTGGGGACAGGGTTAGG(+AAAAAA)GTACCTGCCCCTGGAAAGGCTGCTGCAAGGA NGG 10 8 17 8 24 0 100 5 10
    240606161724_stb2 TCAGATCCTTGCAGCAGCCTTTCCAGGGGCAG(+AAAAAA)GTACCCTAACCCTGTCCCCAGGCTCAGTCCA NGG 10 8 17 8 24 0 100 5 10