Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606161651_egsz GGTTTTGTAATTTTTTTGGCATGTACGTGCAG(+AAAAAA)GTACCTATTATTATTATTATTTCTGTTTAAA NGG 10 8 17 8 24 0 100 5 10
    240606161649_qxxu AGGAATTTAAACAGAAATAATAATAATAATAG(+AAAAAA)GTACCTGCACGTACATGCCAAAAAAATTACA NGG 10 8 17 8 24 0 100 5 10
    240606161647_hd3z GCTAGAACAAACCCAGAGGCTTAAACAACAGG(+AAAAAA)GATCCGGTCCCAGGCCACGTGCACAAGATGC NGG 10 8 17 8 24 0 100 5 10
    240606161646_fes9 GAATAGCATCTTGTGCACGTGGCCTGGGACCG(+AAAAAA)GATCCCTGTTGTTTAAGCCTCTGGGTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240606161644_qdr5 TCTGAAAAGCTTCAGGATCAGCCTAATCTCCG(+AAAAAA)GATCCTTATTAAGTGCATCTGCGAGGTCCCG NGG 10 8 17 8 24 0 100 5 10
    240606161642_5a6t GTAGCCGGGACCTCGCAGATGCACTTAATAAG(+AAAAAA)GATCCGGAGATTAGGCTGATCCTGAAGCTTT NGG 10 8 17 8 24 0 100 5 10
    240606161641_ytu8 GTGTGCTTTCACAGAGCGCTCCTCCCTCGTTG(+AAAAAA)GTACCCTGGCCACTTCCTCTGCACAGTACAG NGG 10 8 17 8 24 0 100 5 10
    240606161639_tkdb GAGCTCTGTACTGTGCAGAGGAAGTGGCCAGG(+AAAAAA)GTACCAACGAGGGAGGAGCGCTCTGTGAAAG NGG 10 8 17 8 24 0 100 5 10
    240606161636_xng0 AGTCAGTAAGAATGACCCTGCTGTGCCCCAGG(+AAAAAA)GTACCCATTCCCCACAGCATGCTTATGCCAT NGG 10 8 17 8 24 0 100 5 10
    240606161634_gf2s GTCTCATGGCATAAGCATGCTGTGGGGAATGG(+AAAAAA)GTACCCTGGGGCACAGCAGGGTCATTCTTAC NGG 10 8 17 8 24 0 100 5 10