Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606161723_hlvp AGAGCAATGTCTGATTTCACAACTTTTTCACG(+AAAAAA)GATCCCTGAGACCACGATTAAAATCAGGTTT NGG 10 8 17 8 24 0 100 5 10
    240606161721_7ehl TTTTAAAACCTGATTTTAATCGTGGTCTCAGG(+AAAAAA)GATCCGTGAAAAAGTTGTGAAATCAGACATT NGG 10 8 17 8 24 0 100 5 10
    240606161720_85cr AGTTCTGTGGCTGTGCTCATCTGTGTCATGGG(+AAAAAA)GATCCCCCACAACCTTTGACTTTCTTTGGAG NGG 10 8 17 8 24 0 100 5 10
    240606161718_gaoc AAATGCTCCAAAGAAAGTCAAAGGTTGTGGGG(+AAAAAA)GATCCCCATGACACAGATGAGCACAGCCACA NGG 10 8 17 8 24 0 100 5 10
    240606161716_hnsj ATGGACCAAGGGTTTCATGGGGCAGAAGGAGG(+AAAAAA)GTACCCAGAATTCCCAGCCCTCATCTGGTCC NGG 10 8 17 8 24 0 100 5 10
    240606161714_fels GGCTGGGACCAGATGAGGGCTGGGAATTCTGG(+AAAAAA)GTACCCTCCTTCTGCCCCATGAAACCCTTGG NGG 10 8 17 8 24 0 100 5 10
    240606161713_cnpf CCATTGCTTCCCCTTATAAGATGTCCCTGCAG(+AAAAAA)GTACCAGAGCCAGTGACAGCACACACACAGG NGG 10 8 17 8 24 0 100 5 10
    240606161712_3m3k AGGGTCCTGTGTGTGTGCTGTCACTGGCTCTG(+AAAAAA)GTACCTGCAGGGACATCTTATAAGGGGAAGC NGG 10 8 17 8 24 0 100 5 10
    240606161710_c4ht GGCGTACCCAGGCCAGCACCAGAGGGCGGCAG(+AAAAAA)GTACCCCAGGGAGTGGCTAGGGAGGTTGGTC NGG 10 8 17 8 24 0 100 5 10
    240606161708_upeg CCTTCGACCAACCTCCCTAGCCACTCCCTGGG(+AAAAAA)GTACCTGCCGCCCTCTGGTGCTGGCCTGGGT NGG 10 8 17 8 24 0 100 5 10