Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606161618_7vxs GAGAAAGAGCGGAGTCTGTGGTAGGAAGAACG(+AAAAAA)GTACCCTGTTTGCCTTCAAGTGTGAGTGTTT NGG 10 8 17 8 24 0 100 5 10
    240606161617_l0sv CGCTCAAACACTCACACTTGAAGGCAAACAGG(+AAAAAA)GTACCGTTCTTCCTACCACAGACTCCGCTCT NGG 10 8 17 8 24 0 100 5 10
    240606161616_1x38 CTCGCACACTGCAGGGTCCTTGCCCAGATGAG(+AAAAAA)GTACCAAAGAGGAGCTGGCCCAGATGTGAAT NGG 10 8 17 8 24 0 100 5 10
    240606161614_ub10 AAACGATTCACATCTGGGCCAGCTCCTCTTTG(+AAAAAA)GTACCTCATCTGGGCAAGGACCCTGCAGTGT NGG 10 8 17 8 24 0 100 5 10
    240606161613_msh7 GTGTGTGTGCGCGCGCGCGCGCATGAATGCAG(+AAAAAA)GTACCCATGGAGGCCCTAAGAAGGCATCAGA NGG 10 8 17 8 24 0 100 5 10
    240606161611_rd3k GGGGATCTGATGCCTTCTTAGGGCCTCCATGG(+AAAAAA)GTACCTGCATTCATGCGCGCGCGCGCGCACA NGG 10 8 17 8 24 0 100 5 10
    240606161609_zna3 GGGATTCTCGGAGCCAGCCCTTCCTTACCCTG(+AAAAAA)GTACCCTATCCCCACCCCACTGTCTTCCTGC NGG 10 8 17 8 24 0 100 5 10
    240606161607_zadx ACTTGGCAGGAAGACAGTGGGGTGGGGATAGG(+AAAAAA)GTACCAGGGTAAGGAAGGGCTGGCTCCGAGA NGG 10 8 17 8 24 0 100 5 10
    240606161606_p22s ACAACTCCCTTGGATGAAGTTATAATCACAGG(+AAAAAA)GATCCATTTTTTTTTAAGTTTAAAAGTCATG NGG 10 8 17 8 24 0 100 5 10
    240606161605_rulk AAATTCATGACTTTTAAACTTAAAAAAAAATG(+AAAAAA)GATCCCTGTGATTATAACTTCATCCAAGGGA NGG 10 8 17 8 24 0 100 5 10