Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606161549_otzr TGGTCCACAAGCTGCAAGCTCCCTCCGCATAG(+AAAAAA)GTACCTGTCTGAGGAGCCTTGTCCCTGAGGC NGG 10 8 17 8 24 0 100 5 10
    240606161548_cqbg AGGCTGCCTCAGGGACAAGGCTCCTCAGACAG(+AAAAAA)GTACCTATGCGGAGGGAGCTTGCAGCTTGTG NGG 10 8 17 8 24 0 100 5 10
    240606161547_zej7 AGTGGCTTTCTCTGTGGGCCAGAACTCTTAGG(+AAAAAA)GTACCAGAGCCTCCTGAGTGTGAGCAGAAGA NGG 10 8 17 8 24 0 100 5 10
    240606161545_z50o CTTTCTCTTCTGCTCACACTCAGGAGGCTCTG(+AAAAAA)GTACCCTAAGAGTTCTGGCCCACAGAGAAAG NGG 10 8 17 8 24 0 100 5 10
    240606161544_xy4l TTTTTAAAGAAATATATAGTATTTTTATTTAG(+AAAAAA)GTACCTCCAAACTTGAACTCGTCTGTGTGAA NGG 10 8 17 8 24 0 100 5 10
    240606161543_ugdw AATGCTTCACACAGACGAGTTCAAGTTTGGAG(+AAAAAA)GTACCTAAATAAAAATACTATATATTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240606161541_48i1 GGAGCTAAGCAATTTGGTCCAGTGGAAACTGG(+AAAAAA)GATCCTTGGAGAATTGGTAAGCCCCAGGCCA NGG 10 8 17 8 24 0 100 5 10
    240606161539_cpcr CTTACTGGCCTGGGGCTTACCAATTCTCCAAG(+AAAAAA)GATCCCAGTTTCCACTGGACCAAATTGCTTA NGG 10 8 17 8 24 0 100 5 10
    240606161537_hkue GGTACAAAAAGCTGAGTGTGTTCTCAGGCAGG(+AAAAAA)GATCCTCCAGGACCAGGTGAGGAAGAATTTG NGG 10 8 17 8 24 0 100 5 10
    240606161536_oy97 AGATTCAAATTCTTCCTCACCTGGTCCTGGAG(+AAAAAA)GATCCCTGCCTGAGAACACACTCAGCTTTTT NGG 10 8 17 8 24 0 100 5 10