Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606161603_7ehs CTTGTTGGGGGAGGGACACTCAGACGGGCTTG(+AAAAAA)GTACCTTTAAAGCAGCAATTGTCACTTCTTG NGG 10 8 17 8 24 0 100 5 10
    240606161602_sfst CTAGGCAAGAAGTGACAATTGCTGCTTTAAAG(+AAAAAA)GTACCAAGCCCGTCTGAGTGTCCCTCCCCCA NGG 10 8 17 8 24 0 100 5 10
    240606161601_0d5g CTCTTTCCGCCATCCGACCCCTATGGGATGAG(+AAAAAA)GATCCTCGGAGGCCAGGCCTGTGGAGAGGTC NGG 10 8 17 8 24 0 100 5 10
    240606161559_o2tm TCCCAGACCTCTCCACAGGCCTGGCCTCCGAG(+AAAAAA)GATCCTCATCCCATAGGGGTCGGATGGCGGA NGG 10 8 17 8 24 0 100 5 10
    240606161558_mr99 GTATGAGTGGCTCTGAAAAGCCATAAGGATAG(+AAAAAA)GTACCTACATGAGAAGGACACCTTTCCTTTC NGG 10 8 17 8 24 0 100 5 10
    240606161557_qcpf CTAGCGAAAGGAAAGGTGTCCTTCTCATGTAG(+AAAAAA)GTACCTATCCTTATGGCTTTTCAGAGCCACT NGG 10 8 17 8 24 0 100 5 10
    240606161555_px3n GGAGTTTTTATACGTGCATATGCATAAGCAGG(+AAAAAA)GATCCTTGGGTTACATGACAGGGGAGGTGAC NGG 10 8 17 8 24 0 100 5 10
    240606161553_pw4w CTCTTGTCACCTCCCCTGTCATGTAACCCAAG(+AAAAAA)GATCCCTGCTTATGCATATGCACGTATAAAA NGG 10 8 17 8 24 0 100 5 10
    240606161552_6qo4 ACAGATACAAATCTGAATTGTGTAAGACTCAG(+AAAAAA)GATCCTGCTTCCAGCCCTGTGTGTAGGTTGT NGG 10 8 17 8 24 0 100 5 10
    240606161550_eqrm TGCTGACAACCTACACACAGGGCTGGAAGCAG(+AAAAAA)GATCCTGAGTCTTACACAATTCAGATTTGTA NGG 10 8 17 8 24 0 100 5 10