Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606161535_jjcn TCAAAACACAAGGTCGGGGGCAATAGGAGATG(+AAAAAA)GTACCTGATGCCAGTCTCATCTCCAACTGCA NGG 10 8 17 8 24 0 100 5 10
    240606161533_d89w ACATGTGCAGTTGGAGATGAGACTGGCATCAG(+AAAAAA)GTACCATCTCCTATTGCCCCCGACCTTGTGT NGG 10 8 17 8 24 0 100 5 10
    240606161532_v17w CAAGGTAGAGTGTTCATACCTGGGGAAAGCAG(+AAAAAA)GTACCCTCTGGGTCTACATGGGGAGAGAGAG NGG 10 8 17 8 24 0 100 5 10
    240606161530_cukn GCTCTCTCTCTCTCCCCATGTAGACCCAGAGG(+AAAAAA)GTACCTGCTTTCCCCAGGTATGAACACTCTA NGG 10 8 17 8 24 0 100 5 10
    240606161529_m8ij CACAGGCTGCTCCCCACCTCAGTTCTATAAAG(+AAAAAA)GATCCAGACAGGAGAGGCCAAGAGAGAGAGA NGG 10 8 17 8 24 0 100 5 10
    240606161528_et1i GCCTCTCTCTCTCTCTTGGCCTCTCCTGTCTG(+AAAAAA)GATCCTTTATAGAACTGAGGTGGGGAGCAGC NGG 10 8 17 8 24 0 100 5 10
    240606161526_2h62 TCCCAAAGAATGAAGCAATCTATGAAGAGAGG(+AAAAAA)GTACCAGACAAAGATCCAAGACACCAGCCCA NGG 10 8 17 8 24 0 100 5 10
    240606161525_7rlm GGATTTGGGCTGGTGTCTTGGATCTTTGTCTG(+AAAAAA)GTACCCTCTCTTCATAGATTGCTTCATTCTT NGG 10 8 17 8 24 0 100 5 10
    240606161524_plsz CATTACTTTTTTCCTGTATATGAAGAAAATTG(+AAAAAA)GTACCCTGTACTTACAATTTATTTATGTTAT NGG 10 8 17 8 24 0 100 5 10
    240606161522_yfhz ATGCAATAACATAAATAAATTGTAAGTACAGG(+AAAAAA)GTACCAATTTTCTTCATATACAGGAAAAAAG NGG 10 8 17 8 24 0 100 5 10