Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606161433_xtfm GAGTAAATAGGGGACATGGTGCATTTTTGCTG(+AAAAAA)GATCCTGTGAAAGGTTTATTTAGAGCCGATT NGG 10 8 17 8 24 0 100 5 10
    240606161432_3nsq TCTGGAATCGGCTCTAAATAAACCTTTCACAG(+AAAAAA)GATCCAGCAAAAATGCACCATGTCCCCTATT NGG 10 8 17 8 24 0 100 5 10
    240606161431_1avw TAGTTTTGAGGGCTTACACAAGCTTATTCTTG(+AAAAAA)GTACCACAGGAATTTGGTTAAATGGAATTTC NGG 10 8 17 8 24 0 100 5 10
    240606161429_2ey1 CAAAGGAAATTCCATTTAACCAAATTCCTGTG(+AAAAAA)GTACCAAGAATAAGCTTGTGTAAGCCCTCAA NGG 10 8 17 8 24 0 100 5 10
    240606161428_vlik GCATTCCCCGCTGCAGTCACCTCATAGGCTTG(+AAAAAA)GATCCATCAAGACCTACTTCAGGCCCCGGCT NGG 10 8 17 8 24 0 100 5 10
    240606161427_ydnd TGGAGAGCCGGGGCCTGAAGTAGGTCTTGATG(+AAAAAA)GATCCAAGCCTATGAGGTGACTGCAGCGGGG NGG 10 8 17 8 24 0 100 5 10
    240606161424_9dft AGCGCACAGGGAACAGAGGGAGGCCCACCAGG(+AAAAAA)GATCCTGGCTTGCCTACCGAAGGCTATTGCT NGG 10 8 17 8 24 0 100 5 10
    240606161422_qpwm ACAGCAGCAATAGCCTTCGGTAGGCAAGCCAG(+AAAAAA)GATCCCTGGTGGGCCTCCCTCTGTTCCCTGT NGG 10 8 17 8 24 0 100 5 10
    240606161421_fp3d CCGATATTGGATGTGGGCCATTTATGGTGTTG(+AAAAAA)GTACCCACACCAGTTGCCCACTTAACGCCCC NGG 10 8 17 8 24 0 100 5 10
    240606161420_hjh4 GCGGGGGGGCGTTAAGTGGGCAACTGGTGTGG(+AAAAAA)GTACCAACACCATAAATGGCCCACATCCAAT NGG 10 8 17 8 24 0 100 5 10