Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606161342_ag27 CATTCCTGCCAGTTTGGCTAGCTCTAGATAGG(+AAAAAA)GATCCTGCCTTGTATCCTGAGACCCAGAGGA NGG 10 8 17 8 24 0 100 5 10
    240606161341_nams TCTGGTCCTCTGGGTCTCAGGATACAAGGCAG(+AAAAAA)GATCCCTATCTAGAGCTAGCCAAACTGGCAG NGG 10 8 17 8 24 0 100 5 10
    240606161339_hamu GAAGGAATCATACTAGAGAAGAAAAGTCAGAG(+AAAAAA)GTACCACAATCAATAAGAAAACAGTAAATTA NGG 10 8 17 8 24 0 100 5 10
    240606161338_7hm1 GTTTGTAATTTACTGTTTTCTTATTGATTGTG(+AAAAAA)GTACCTCTGACTTTTCTTCTCTAGTATGATT NGG 10 8 17 8 24 0 100 5 10
    240606161337_rhky GGGCGCTAACCCTCCCACCACTCGGCAGCGAG(+AAAAAA)GATCCATGGTGGCTTTCATCATCTTTCATGG NGG 10 8 17 8 24 0 100 5 10
    240606161335_t2x4 TGTCCCCATGAAAGATGATGAAAGCCACCATG(+AAAAAA)GATCCTCGCTGCCGAGTGGTGGGAGGGTTAG NGG 10 8 17 8 24 0 100 5 10
    240606161334_m0a8 GTTAGAATCACAACCAACTCTGTAATAAATGG(+AAAAAA)GATCCTTTAACTCCTGACCTACTAAAGCCCC NGG 10 8 17 8 24 0 100 5 10
    240606161332_7yzb TCTTGGGGGCTTTAGTAGGTCAGGAGTTAAAG(+AAAAAA)GATCCCATTTATTACAGAGTTGGTTGTGATT NGG 10 8 17 8 24 0 100 5 10
    240606161331_x2lx ATGCGTGAGAACCCAATGTGAGAACAGGGAAG(+AAAAAA)GATCCTTTCTGATTGTTGTGGCCTTGGGTTT NGG 10 8 17 8 24 0 100 5 10
    240606161330_b9a8 AAATAAAACCCAAGGCCACAACAATCAGAAAG(+AAAAAA)GATCCTTCCCTGTTCTCACATTGGGTTCTCA NGG 10 8 17 8 24 0 100 5 10