Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606161259_cqns CTCTAACCTATGACTCTAATAACATCATAATG(+AAAAAA)GATCCCAAATCCTTATGCTCAGGAGGCCCAG NGG 10 8 17 8 24 0 100 5 10
    240606161257_lxxu ATCTGCTGGGCCTCCTGAGCATAAGGATTTGG(+AAAAAA)GATCCATTATGATGTTATTAGAGTCATAGGT NGG 10 8 17 8 24 0 100 5 10
    240606161256_ua34 TTACCGTCACCCTCTCAGAACCCTCCCTGCTG(+AAAAAA)GTACCTACCCTCTATTTATTAGCCGACTCGT NGG 10 8 17 8 24 0 100 5 10
    240606161255_jikw CAGGGACGAGTCGGCTAATAAATAGAGGGTAG(+AAAAAA)GTACCAGCAGGGAGGGTTCTGAGAGGGTGAC NGG 10 8 17 8 24 0 100 5 10
    240606161253_0hs2 TGGCTCAGTAGACCCAAACCAGGACTGTCCTG(+AAAAAA)GATCCACTTTAGTCATGCTACCAGTGGGCTG NGG 10 8 17 8 24 0 100 5 10
    240606161252_kiz8 TGTAGCAGCCCACTGGTAGCATGACTAAAGTG(+AAAAAA)GATCCAGGACAGTCCTGGTTTGGGTCTACTG NGG 10 8 17 8 24 0 100 5 10
    240606161250_cuo9 TCAAAAAAGCCCCAAGAAATTTTGAAGTGTGG(+AAAAAA)GTACCTCGTTGATTCTTACGCCAGGGCACAG NGG 10 8 17 8 24 0 100 5 10
    240606161249_f1ke TGATCCTGTGCCCTGGCGTAAGAATCAACGAG(+AAAAAA)GTACCCACACTTCAAAATTTCTTGGGGCTTT NGG 10 8 17 8 24 0 100 5 10
    240606161248_41uc CATTTTCCAGTATAATAAAGGTAGCTAAGATG(+AAAAAA)GATCCATATGCAGAAATAGATTACAGTCCTT NGG 10 8 17 8 24 0 100 5 10
    240606161247_n3ap TGGCTAAGGACTGTAATCTATTTCTGCATATG(+AAAAAA)GATCCATCTTAGCTACCTTTATTATACTGGA NGG 10 8 17 8 24 0 100 5 10