Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606161229_q8o4 GTGTGGCCAGTGCCAAGACCACCGTCTCTTGG(+AAAAAA)GATCCTCTGTCTTTATCTTTTGTTCGTCTGT NGG 10 8 17 8 24 0 100 5 10
    240606161228_mhgq ATGAAACAGACGAACAAAAGATAAAGACAGAG(+AAAAAA)GATCCCAAGAGACGGTGGTCTTGGCACTGGC NGG 10 8 17 8 24 0 100 5 10
    240606161227_87fs CCCGAGCCCTTCACGGGCATCCGAGGGAAAGG(+AAAAAA)GTACCAGAAGCATTCCCTCTTAATCATTGCA NGG 10 8 17 8 24 0 100 5 10
    240606161225_dszq ATGTCTGCAATGATTAAGAGGGAATGCTTCTG(+AAAAAA)GTACCCTTTCCCTCGGATGCCCGTGAAGGGC NGG 10 8 17 8 24 0 100 5 10
    240606161224_qplp GAGAACAGAACAGAACTTCAGAGATCAAAGTG(+AAAAAA)GATCCGAGGGTAGATGTACCTTAGCCCAGAG NGG 10 8 17 8 24 0 100 5 10
    240606161222_1a0z TTTTACTCTGGGCTAAGGTACATCTACCCTCG(+AAAAAA)GATCCACTTTGATCTCTGAAGTTCTGTTCTG NGG 10 8 17 8 24 0 100 5 10
    240606161220_32kx AATGACCAAGTCAAAGTGTACAGGGTAAGTTG(+AAAAAA)GTACCTCAGCCTGGGCCAAGGAGAGATTCAA NGG 10 8 17 8 24 0 100 5 10
    240606161219_8vh7 ATTACTTGAATCTCTCCTTGGCCCAGGCTGAG(+AAAAAA)GTACCAACTTACCCTGTACACTTTGACTTGG NGG 10 8 17 8 24 0 100 5 10
    240606161218_3gyb GAGCTTGATTACTTGGGGCTTGTTGTGGGAAG(+AAAAAA)GTACCATTGGGGATTGACCTCAGAGCCCCTA NGG 10 8 17 8 24 0 100 5 10
    240606161216_bcyi TACTCTAGGGGCTCTGAGGTCAATCCCCAATG(+AAAAAA)GTACCTTCCCACAACAAGCCCCAAGTAATCA NGG 10 8 17 8 24 0 100 5 10