Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606161142_d7ss TAGGAAGGTGATGCTTAATACTATGTGCCCAG(+AAAAAA)GATCCCTCTGAAGTTTGCTGGAAGGCATGAT NGG 10 8 17 8 24 0 100 5 10
    240606161141_8q2g GGAGAATCATGCCTTCCAGCAAACTTCAGAGG(+AAAAAA)GATCCTGGGCACATAGTATTAAGCATCACCT NGG 10 8 17 8 24 0 100 5 10
    240606161139_iywx ACAGACATCTGTCAGGGGACAGGGAGCTTGGG(+AAAAAA)GTACCTGAACCAGGTCAGCTGATGGAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240606161138_xnw5 AGTCTCTGCTTCCATCAGCTGACCTGGTTCAG(+AAAAAA)GTACCCCAAGCTCCCTGTCCCCTGACAGATG NGG 10 8 17 8 24 0 100 5 10
    240606161137_cl6l TAAGAATTTACATGCACATCGCTTGACATTCG(+AAAAAA)GTACCCAGAGAATTCTTTATGTGCCTTTCAA NGG 10 8 17 8 24 0 100 5 10
    240606161136_opgx CTCTCTTGAAAGGCACATAAAGAATTCTCTGG(+AAAAAA)GTACCGAATGTCAAGCGATGTGCATGTAAAT NGG 10 8 17 8 24 0 100 5 10
    240606161133_bmuu TGGCTGGCACAGCCCGGTCTCAGGCCCCCTGG(+AAAAAA)GATCCAGACCTCCTCCTTGCACTGAGAATGC NGG 10 8 17 8 24 0 100 5 10
    240606161132_1sl1 AAAGTGCATTCTCAGTGCAAGGAGGAGGTCTG(+AAAAAA)GATCCCAGGGGGCCTGAGACCGGGCTGTGCC NGG 10 8 17 8 24 0 100 5 10
    240606161130_ngvh TGTGTTTCCCTTTAGTCTCCGCCTATACTTGG(+AAAAAA)GATCCTTTCTCACTGCTGGCCATTCAGTGAG NGG 10 8 17 8 24 0 100 5 10
    240606161129_fe11 CCTTCCTCACTGAATGGCCAGCAGTGAGAAAG(+AAAAAA)GATCCCAAGTATAGGCGGAGACTAAAGGGAA NGG 10 8 17 8 24 0 100 5 10