Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606161113_o109 TCCTGCCTGGCTACATGAAGTGGTCACTTGAG(+AAAAAA)GATCCATTCCACCCACTACTAGAAGTCTCAG NGG 10 8 17 8 24 0 100 5 10
    240606161112_85l2 TTTAGCTGAGACTTCTAGTAGTGGGTGGAATG(+AAAAAA)GATCCTCAAGTGACCACTTCATGTAGCCAGG NGG 10 8 17 8 24 0 100 5 10
    240606161110_0777 ATTTCCTAAAACAATTAAGAAATTTTGCTATG(+AAAAAA)GATCCAAGTTTCCTTACCCATAGATCTGACA NGG 10 8 17 8 24 0 100 5 10
    240606161109_g4s3 CATCATGTCAGATCTATGGGTAAGGAAACTTG(+AAAAAA)GATCCATAGCAAAATTTCTTAATTGTTTTAG NGG 10 8 17 8 24 0 100 5 10
    240606161107_6ofk TGTGGGGCATTTCTTATGCCCTGCCCCCAAGG(+AAAAAA)GATCCAAGTCACCACATTGCCAAAGAATCCA NGG 10 8 17 8 24 0 100 5 10
    240606161106_phqn AAGCCTGGATTCTTTGGCAATGTGGTGACTTG(+AAAAAA)GATCCCTTGGGGGCAGGGCATAAGAAATGCC NGG 10 8 17 8 24 0 100 5 10
    240606161105_kfys TGGCCAACAAGGTGGCTGACCCAATCGGTGAG(+AAAAAA)GATCCAAGAGATGGAGGGAGAGCACGAAAGT NGG 10 8 17 8 24 0 100 5 10
    240606161103_dded GTTTTACTTTCGTGCTCTCCCTCCATCTCTTG(+AAAAAA)GATCCTCACCGATTGGGTCAGCCACCTTGTT NGG 10 8 17 8 24 0 100 5 10
    240606161102_4j9b TTAAACCATTTTATGAGTACCATTCTGCAAAG(+AAAAAA)GATCCCATAGTGGTGCACAGCCTTAAGAAGC NGG 10 8 17 8 24 0 100 5 10
    240606161100_t2cs GCCTGGCTTCTTAAGGCTGTGCACCACTATGG(+AAAAAA)GATCCTTTGCAGAATGGTACTCATAAAATGG NGG 10 8 17 8 24 0 100 5 10