Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606161213_7av2 ATGGTGGCTTCAGACTCTCTCCAGTTCCAGGG(+AAAAAA)GATCCAATGCCCCAATACCCTCTCCAGCCAC NGG 10 8 17 8 24 0 100 5 10
    240606161211_se31 GGAGAGTGGCTGGAGAGGGTATTGGGGCATTG(+AAAAAA)GATCCCCTGGAACTGGAGAGAGTCTGAAGCC NGG 10 8 17 8 24 0 100 5 10
    240606161210_qc59 AGTGTAGGACTGGAGACTGAGAAGGGGTTTGG(+AAAAAA)GTACCCGCGCTCTTCCGAGGAAGTGCTGTTA NGG 10 8 17 8 24 0 100 5 10
    240606161208_qexz TCTCTTAACAGCACTTCCTCGGAAGAGCGCGG(+AAAAAA)GTACCCAAACCCCTTCTCAGTCTCCAGTCCT NGG 10 8 17 8 24 0 100 5 10
    240606161207_at1j GCTTGCTCCGTTGCGCACTTTGGATCTCTGGG(+AAAAAA)GATCCCTTTCTTCTTGGCTCACTGAGATGAT NGG 10 8 17 8 24 0 100 5 10
    240606161205_h276 CCTACATCATCTCAGTGAGCCAAGAAGAAAGG(+AAAAAA)GATCCCCAGAGATCCAAAGTGCGCAACGGAG NGG 10 8 17 8 24 0 100 5 10
    240606161204_hu2e GGCTCAACGCTGGAATCAGTTTCCCTGAAAAG(+AAAAAA)GATCCTCGGACTAAAAGTGGCATGTTAGAAA NGG 10 8 17 8 24 0 100 5 10
    240606161202_ma49 GGTTTTTTCTAACATGCCACTTTTAGTCCGAG(+AAAAAA)GATCCTTTTCAGGGAAACTGATTCCAGCGTT NGG 10 8 17 8 24 0 100 5 10
    240606161201_4zn9 GAACGACAACCTGAGAGAAGCAGGTGGACTGG(+AAAAAA)GTACCTTCTGCTCTCCACTGGCCATTGGCAG NGG 10 8 17 8 24 0 100 5 10
    240606161159_4zs5 CACTCCTGCCAATGGCCAGTGGAGAGCAGAAG(+AAAAAA)GTACCCAGTCCACCTGCTTCTCTCAGGTTGT NGG 10 8 17 8 24 0 100 5 10