Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606161313_kr22 AGAGGCTTTCTGTGCTACACTGTCTTAGCTCG(+AAAAAA)GATCCGGTGGGGGTTTGGCTGTCCTGTGGCC NGG 10 8 17 8 24 0 100 5 10
    240606161311_wp9x GGGCAGGCCACAGGACAGCCAAACCCCCACCG(+AAAAAA)GATCCGAGCTAAGACAGTGTAGCACAGAAAG NGG 10 8 17 8 24 0 100 5 10
    240606161310_0f0a CCTGGCTGGAGATGCTGCTGGGAGAGGCTGTG(+AAAAAA)GTACCCGTAGGGAGAATCCCTAGCTCCCACC NGG 10 8 17 8 24 0 100 5 10
    240606161308_c69w GTGGAGGTGGGAGCTAGGGATTCTCCCTACGG(+AAAAAA)GTACCACAGCCTCTCCCAGCAGCATCTCCAG NGG 10 8 17 8 24 0 100 5 10
    240606161307_djvw CCAGGTCCTAAATAGGGAGAGGTCCAGAAATG(+AAAAAA)GATCCCTTGCAGGAAGGCTGCTTCCTCTGGT NGG 10 8 17 8 24 0 100 5 10
    240606161306_e7m5 AGACCACCAGAGGAAGCAGCCTTCCTGCAAGG(+AAAAAA)GATCCATTTCTGGACCTCTCCCTATTTAGGA NGG 10 8 17 8 24 0 100 5 10
    240606161304_ood6 CAAAGCCAAGGTTCATTCGGTGGAAGTTTTAG(+AAAAAA)GTACCTCAAGGCAGTTCTGGAAAGTGTACCC NGG 10 8 17 8 24 0 100 5 10
    240606161303_wf86 AACTGGGGTACACTTTCCAGAACTGCCTTGAG(+AAAAAA)GTACCTAAAACTTCCACCGAATGAACCTTGG NGG 10 8 17 8 24 0 100 5 10
    240606161301_m29g GAATACAAAGACAGCTGCTTGTTTTCTCCTTG(+AAAAAA)GTACCTTAAAAATGAGTAAGTGCCACGTCTG NGG 10 8 17 8 24 0 100 5 10
    240606161300_uciw TTCTCCAGACGTGGCACTTACTCATTTTTAAG(+AAAAAA)GTACCAAGGAGAAAACAAGCAGCTGTCTTTG NGG 10 8 17 8 24 0 100 5 10