Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606161359_5sj4 GTGTATATAAGCAGTCCTCCCTGGGTACCCGG(+AAAAAA)GATCCCTGTAGCAGCATCGAGGCGTTCCTGC NGG 10 8 17 8 24 0 100 5 10
    240606161357_l5t1 TTATTGCAGGAACGCCTCGATGCTGCTACAGG(+AAAAAA)GATCCCGGGTACCCAGGGAGGACTGCTTATA NGG 10 8 17 8 24 0 100 5 10
    240606161356_qg6l CCATCCATGCTGGGTGCCATCCCACCTCAATG(+AAAAAA)GTACCAATATAACCCCCAACAGACATTTATA NGG 10 8 17 8 24 0 100 5 10
    240606161355_fsfm GCCTTTATAAATGTCTGTTGGGGGTTATATTG(+AAAAAA)GTACCATTGAGGTGGGATGGCACCCAGCATG NGG 10 8 17 8 24 0 100 5 10
    240606161352_uvxo CTCCACTCCTTCTCTACTCTTCTGGGCCCCAG(+AAAAAA)GATCCTCTGCCTAGGGAATGGTGCCATCCAT NGG 10 8 17 8 24 0 100 5 10
    240606161351_g0h7 CCAGCATGGATGGCACCATTCCCTAGGCAGAG(+AAAAAA)GATCCTGGGGCCCAGAAGAGTAGAGAAGGAG NGG 10 8 17 8 24 0 100 5 10
    240606161349_9mdt CAGGCCCAGTGTCACTCTTCTTGCTGCCTTTG(+AAAAAA)GATCCAGATGTGAATTCTCTGCTACCTCTCC NGG 10 8 17 8 24 0 100 5 10
    240606161348_tn25 GTGCTGGAGAGGTAGCAGAGAATTCACATCTG(+AAAAAA)GATCCAAAGGCAGCAAGAAGAGTGACACTGG NGG 10 8 17 8 24 0 100 5 10
    240606161346_bt35 ACTTTTGAGGCCACTGCCAGGACCCCAGCCTG(+AAAAAA)GATCCGGGTTTTATCCAGGGACACCCAGCTA NGG 10 8 17 8 24 0 100 5 10
    240606161344_ear7 CTTCATAGCTGGGTGTCCCTGGATAAAACCCG(+AAAAAA)GATCCAGGCTGGGGTCCTGGCAGTGGCCTCA NGG 10 8 17 8 24 0 100 5 10