Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606161059_b4lm CAGTTACTAAGGCACATGACCGTGGTGAGGAG(+AAAAAA)GATCCTCAGGACCTGCCGGAGCTGCCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240606161057_q41k GGTGGCTGCAGGCAGCTCCGGCAGGTCCTGAG(+AAAAAA)GATCCTCCTCACCACGGTCATGTGCCTTAGT NGG 10 8 17 8 24 0 100 5 10
    240606161056_ik3f CTGTGTTTAGGTGTCACGTGTGAGATAATGTG(+AAAAAA)GTACCAGGAAAGGAGCTATGTACTGAGGCTT NGG 10 8 17 8 24 0 100 5 10
    240606161054_mz2j GAAACAAGCCTCAGTACATAGCTCCTTTCCTG(+AAAAAA)GTACCACATTATCTCACACGTGACACCTAAA NGG 10 8 17 8 24 0 100 5 10
    240606161053_98gi CAGCACAGTAGAGTCACCGAGGGGTATGGTAG(+AAAAAA)GTACCAGAGGAACTATTCAGTCCTCATGGAA NGG 10 8 17 8 24 0 100 5 10
    240606161051_5n5u GTCTGTTCCATGAGGACTGAATAGTTCCTCTG(+AAAAAA)GTACCTACCATACCCCTCGGTGACTCTACTG NGG 10 8 17 8 24 0 100 5 10
    240606161050_3xea CTCTCAATCGGGGCTGGAAAAGAACAAAGTGG(+AAAAAA)GATCCCACACTTCTCCTGCACCCTCCAGCAG NGG 10 8 17 8 24 0 100 5 10
    240606161048_grh5 GCAACCTGCTGGAGGGTGCAGGAGAAGTGTGG(+AAAAAA)GATCCCACTTTGTTCTTTTCCAGCCCCGATT NGG 10 8 17 8 24 0 100 5 10
    240606161047_wpp5 GCCATCCATGGAATGAGTTTATGTGTACAAAG(+AAAAAA)GATCCAGAAAAGGGGACATTTTAAAAAAATC NGG 10 8 17 8 24 0 100 5 10
    240606161046_23an ACAGTGATTTTTTTAAAATGTCCCCTTTTCTG(+AAAAAA)GATCCTTTGTACACATAAACTCATTCCATGG NGG 10 8 17 8 24 0 100 5 10