Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606162326_s51l ATTGTTAATGTGCCTTGCAACTGGGAGTCTAG(+AAAAAA)GTACCTAATGAAACTTTGTGACTTTAGCACA NGG 10 8 17 8 24 0 100 5 10
    240606162324_ixwe GAACTTGTGCTAAAGTCACAAAGTTTCATTAG(+AAAAAA)GTACCTAGACTCCCAGTTGCAAGGCACATTA NGG 10 8 17 8 24 0 100 5 10
    240606162323_2bc8 GCCTGGCCTGGGTGGGCCTGTGCTGTGCCTTG(+AAAAAA)GTACCATCTTCCCTCGATTTTCCTTGACCCT NGG 10 8 17 8 24 0 100 5 10
    240606162321_a64n GAAGGAGGGTCAAGGAAAATCGAGGGAAGATG(+AAAAAA)GTACCAAGGCACAGCACAGGCCCACCCAGGC NGG 10 8 17 8 24 0 100 5 10
    240606162320_s7qt CATGCATATTATTTATTTCTGTGCAAACTGTG(+AAAAAA)GATCCGTAACCAAGGTGAAGAAAGTTGGGAT NGG 10 8 17 8 24 0 100 5 10
    240606162319_1esg CAGAAATCCCAACTTTCTTCACCTTGGTTACG(+AAAAAA)GATCCACAGTTTGCACAGAAATAAATAATAT NGG 10 8 17 8 24 0 100 5 10
    240606162317_u6s6 CTTCACATAGCCACATCATAGACGTTTTTAGG(+AAAAAA)GATCCCTGCTCTCCTAAATTGCTCCCCGAGT NGG 10 8 17 8 24 0 100 5 10
    240606162316_q5a5 TACTGACTCGGGGAGCAATTTAGGAGAGCAGG(+AAAAAA)GATCCCTAAAAACGTCTATGATGTGGCTATG NGG 10 8 17 8 24 0 100 5 10
    240606162314_47ue CCTAACTCTGAGCAAGTGACTTCTGCAAAAGG(+AAAAAA)GTACCCCGAAGGAAGCCATGTGGGCATCCTG NGG 10 8 17 8 24 0 100 5 10
    240606162312_0pc3 CTGGACAGGATGCCCACATGGCTTCCTTCGGG(+AAAAAA)GTACCCTTTTGCAGAAGTCACTTGCTCAGAG NGG 10 8 17 8 24 0 100 5 10