Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613180640_2ol6 TTTTTTAAAGGGGTGGGGTGTGGTGGGGTGGG(+AGGACA)GTACCAAGATCTGGATTTATGGTGTAAATAT NGG 10 8 17 8 24 0 100 5 10
    240613180638_v7wt GAGCAATATTTACACCATAAATCCAGATCTTG(+AGGACA)GTACCCCACCCCACCACACCCCACCCCTTTA NGG 10 8 17 8 24 0 100 5 10
    240613180637_1ibo CATAAAGTGACAGGAGCCTGGGTAAAGACTTG(+AGGACA)GTACCAACATTGGATGCTGTCAAGGCTCTCC NGG 10 8 17 8 24 0 100 5 10
    240613180636_t0gf GCCAGGGAGAGCCTTGACAGCATCCAATGTTG(+AGGACA)GTACCAAGTCTTTACCCAGGCTCCTGTCACT NGG 10 8 17 8 24 0 100 5 10
    240613180635_s6cz ACCGGTGAAGAAAGAAGTCTCTCTTCAACACG(+AGGACA)GATCCAGGCAGCCAGTCTTCACCTGAGCACA NGG 10 8 17 8 24 0 100 5 10
    240613180633_w4x1 CACCCTGTGCTCAGGTGAAGACTGGCTGCCTG(+AGGACA)GATCCGTGTTGAAGAGAGACTTCTTTCTTCA NGG 10 8 17 8 24 0 100 5 10
    240613180632_51rt TTTATATATATTAACTTTCATTGCATTTTTTG(+AGGACA)GTACCTTCACAACTGGTGTGAAATAAATTCA NGG 10 8 17 8 24 0 100 5 10
    240613180631_hmtv TGATGTGAATTTATTTCACACCAGTTGTGAAG(+AGGACA)GTACCAAAAAATGCAATGAAAGTTAATATAT NGG 10 8 17 8 24 0 100 5 10
    240613180629_jhzu CAATAAAGGAACAAAAGAACCCAGCAATCCAG(+AGGACA)GTACCATGATCTTGCTCAAGTAGAAGAGATG NGG 10 8 17 8 24 0 100 5 10
    240613180628_ku8a GAGGGCATCTCTTCTACTTGAGCAAGATCATG(+AGGACA)GTACCTGGATTGCTGGGTTCTTTTGTTCCTT NGG 10 8 17 8 24 0 100 5 10