Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613180803_2mmg ATCTATGAACATGTTTGGTTGGGATGTGTTTG(+AGGACA)GATCCAGGACCTGGGTCCCCTGGGAAATGTT NGG 10 8 17 8 24 0 100 5 10
    240613180801_h7ae GCCCTAACATTTCCCAGGGGACCCAGGTCCTG(+AGGACA)GATCCAAACACATCCCAACCAAACATGTTCA NGG 10 8 17 8 24 0 100 5 10
    240613180759_gai5 TAGACTCCCTCTGGGAGCTCTTGGTGGCTTGG(+AGGACA)GTACCCTGACCTGGCCTTGGGGACTCCTGAG NGG 10 8 17 8 24 0 100 5 10
    240613180757_k5fs CAGTGCTCAGGAGTCCCCAAGGCCAGGTCAGG(+AGGACA)GTACCCAAGCCACCAAGAGCTCCCAGAGGGA NGG 10 8 17 8 24 0 100 5 10
    240613180754_hrve TTTTGGAACTGTTGCGCTGTGCCCTGGGGGGG(+AGGACA)GATCCCCGCTCAGACTGGCCTCCCACACCCA NGG 10 8 17 8 24 0 100 5 10
    240613180752_e7j5 TCATCTGGGTGTGGGAGGCCAGTCTGAGCGGG(+AGGACA)GATCCCCCCCCAGGGCACAGCGCAACAGTTC NGG 10 8 17 8 24 0 100 5 10
    240613180751_07hc GCCCTGGATGGATGACTCTGGAATCCAGTATG(+AGGACA)GTACCATGGAAGGAGCACTGGACTGAGAGCC NGG 10 8 17 8 24 0 100 5 10
    240613180749_3wxn TCCAGGGCTCTCAGTCCAGTGCTCCTTCCATG(+AGGACA)GTACCATACTGGATTCCAGAGTCATCCATCC NGG 10 8 17 8 24 0 100 5 10
    240613180747_fuev AGGAAAGTATGGTTTCCCCAGGTCTGAACTGG(+AGGACA)GTACCCCCAGGGTGTTTACTAACTCTGGTGA NGG 10 8 17 8 24 0 100 5 10
    240613180745_qe8x ACCCTTCACCAGAGTTAGTAAACACCCTGGGG(+AGGACA)GTACCCAGTTCAGACCTGGGGAAACCATACT NGG 10 8 17 8 24 0 100 5 10