Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613180525_c2ic TTTATGTGTATTGTATACCACATGTGTGCCTG(+AGGACA)GTACCTGTGAAGGTCAGAAGAGGCCATCAGC NGG 10 8 17 8 24 0 100 5 10
    240613180523_g2to GTAGAGCTGATGGCCTCTTCTGACCTTCACAG(+AGGACA)GTACCAGGCACACATGTGGTATACAATACAC NGG 10 8 17 8 24 0 100 5 10
    240613180522_taxp TGTATTCGTTGTATGTGTGTACACACAGTCTG(+AGGACA)GTACCTGACATTCAACAGTGACTCTTTATAG NGG 10 8 17 8 24 0 100 5 10
    240613180521_9o2w CCAGGCTATAAAGAGTCACTGTTGAATGTCAG(+AGGACA)GTACCAGACTGTGTGTACACACATACAACGA NGG 10 8 17 8 24 0 100 5 10
    240613180520_dnv9 ATAGTGCCCCTGTGGCCCAGAAGAGAGTGTTG(+AGGACA)GATCCCTGAGACTGGAGTTATAGACAGAAGT NGG 10 8 17 8 24 0 100 5 10
    240613180518_xo3z AGTTCACTTCTGTCTATAACTCCAGTCTCAGG(+AGGACA)GATCCAACACTCTCTTCTGGGCCACAGGGGC NGG 10 8 17 8 24 0 100 5 10
    240613180516_2sx0 TCTGGTTAGTATAAGTCAAATCAGGGTATTTG(+AGGACA)GTACCTGTCTTGCTGTACTGAATTATAGCTA NGG 10 8 17 8 24 0 100 5 10
    240613180515_0ub3 ATTATTAGCTATAATTCAGTACAGCAAGACAG(+AGGACA)GTACCAAATACCCTGATTTGACTTATACTAA NGG 10 8 17 8 24 0 100 5 10
    240613180513_ef0o CACTGCCTGTGCTGCTAGGATTTAGTTATAAG(+AGGACA)GATCCAGTACTCCAGGGCCAGGGCCAGTGTA NGG 10 8 17 8 24 0 100 5 10
    240613180512_pbk8 CTCTGTACACTGGCCCTGGCCCTGGAGTACTG(+AGGACA)GATCCTTATAACTAAATCCTAGCAGCACAGG NGG 10 8 17 8 24 0 100 5 10