Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613180425_8rsa GAATGAGATTTTGTATTTTCCCTTGAGAGATG(+AGGACA)GTACCAAGCAGGCAAGCAACCATGTACCTAC NGG 10 8 17 8 24 0 100 5 10
    240613180424_sdtw AACTGGTAGGTACATGGTTGCTTGCCTGCTTG(+AGGACA)GTACCATCTCTCAAGGGAAAATACAAAATCT NGG 10 8 17 8 24 0 100 5 10
    240613180423_tv9b AATTGGGTTTTGTCTTCTTTAAAAACAAATAG(+AGGACA)GTACCGTAAGCATTTTCTGCTGACGACTGCC NGG 10 8 17 8 24 0 100 5 10
    240613180422_plbo CTTAAGGCAGTCGTCAGCAGAAAATGCTTACG(+AGGACA)GTACCTATTTGTTTTTAAAGAAGACAAAACC NGG 10 8 17 8 24 0 100 5 10
    240613180420_10vp TGAATAAGAACCAAACTGAGTATTATAGTGAG(+AGGACA)GATCCTTGACCACTTAGTAGACCTCAGCAAA NGG 10 8 17 8 24 0 100 5 10
    240613180419_zp82 AGAATTTTGCTGAGGTCTACTAAGTGGTCAAG(+AGGACA)GATCCTCACTATAATACTCAGTTTGGTTCTT NGG 10 8 17 8 24 0 100 5 10
    240613180418_mhnw ACCTTCGCAAAAACTTAAATCATGGAATGGCG(+AGGACA)GTACCACGGTAGAACACTGCAGCTGTCGCCA NGG 10 8 17 8 24 0 100 5 10
    240613180416_jlfg AACCCTGGCGACAGCTGCAGTGTTCTACCGTG(+AGGACA)GTACCGCCATTCCATGATTTAAGTTTTTGCG NGG 10 8 17 8 24 0 100 5 10
    240613180413_mkxq GAATCGACCACCATACCACAGTAACGCCCCTG(+AGGACA)GATCCCTGGCTGCCCACCCACTCTAAGGCTA NGG 10 8 17 8 24 0 100 5 10
    240613180411_k73r CAGGATAGCCTTAGAGTGGGTGGGCAGCCAGG(+AGGACA)GATCCAGGGGCGTTACTGTGGTATGGTGGTC NGG 10 8 17 8 24 0 100 5 10