Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613180546_tfrn TGGCATACTTATGCCATATCTGTTTTGATTGG(+AGGACA)GATCCTGGAACCTCCCTTAAATCAAGGATTG NGG 10 8 17 8 24 0 100 5 10
    240613180545_93nk AAGGCCAATCCTTGATTTAAGGGAGGTTCCAG(+AGGACA)GATCCCAATCAAAACAGATATGGCATAAGTA NGG 10 8 17 8 24 0 100 5 10
    240613180543_duye GCCACTTCTGAGCTGAGCACCTGTCTGAGGAG(+AGGACA)GATCCTGACTAGACAAGGAATTCATAAGCCA NGG 10 8 17 8 24 0 100 5 10
    240613180541_dni1 CTCTCTGGCTTATGAATTCCTTGTCTAGTCAG(+AGGACA)GATCCTCCTCAGACAGGTGCTCAGCTCAGAA NGG 10 8 17 8 24 0 100 5 10
    240613180540_3w0r GGAGATGTGTCTCTGGGTGGATGAGCTATTTG(+AGGACA)GTACCTATAACCTGTGTGGCTCTGTAATATA NGG 10 8 17 8 24 0 100 5 10
    240613180539_1wl1 CCCCTTATATTACAGAGCCACACAGGTTATAG(+AGGACA)GTACCAAATAGCTCATCCACCCAGAGACACA NGG 10 8 17 8 24 0 100 5 10
    240613180538_4vlr GGTTTCTGCCCATATGGCTATGTTATCATCAG(+AGGACA)GATCCTGGAGCTTCTATCTGAATGACATAGG NGG 10 8 17 8 24 0 100 5 10
    240613180536_w62v TCTGACCTATGTCATTCAGATAGAAGCTCCAG(+AGGACA)GATCCTGATGATAACATAGCCATATGGGCAG NGG 10 8 17 8 24 0 100 5 10
    240613180534_v0a2 CCTGCTGTGGTGAGCTTAAGGACCCAGGTTCG(+AGGACA)GATCCCCAGTACCCATGTACAATGTTAGCCA NGG 10 8 17 8 24 0 100 5 10
    240613180532_5n34 CACCATGGCTAACATTGTACATGGGTACTGGG(+AGGACA)GATCCGAACCTGGGTCCTTAAGCTCACCACA NGG 10 8 17 8 24 0 100 5 10