Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613180250_zxkd TGCGATGCATTACATTAGCCGAGGCACTTCAG(+AGGACA)GATCCTTCTTATACCCATTCTCTCAACAACT NGG 10 8 17 8 24 0 100 5 10
    240613180249_8f5k ACAAAAGTTGTTGAGAGAATGGGTATAAGAAG(+AGGACA)GATCCTGAAGTGCCTCGGCTAATGTAATGCA NGG 10 8 17 8 24 0 100 5 10
    240613180247_5qa0 TATCAGCTTCATAGATTTCCGTCTTATGTGGG(+AGGACA)GATCCTGGAGAGTTAACGGTCTCATTTCATT NGG 10 8 17 8 24 0 100 5 10
    240613180245_ezwg TGTAGAATGAAATGAGACCGTTAACTCTCCAG(+AGGACA)GATCCCCACATAAGACGGAAATCTATGAAGC NGG 10 8 17 8 24 0 100 5 10
    240613180243_flne TCTCTTCTTGGGACTTCCCGGAGTTTGGGAAG(+AGGACA)GTACCCTAAACTACTCTGAGTCTTTGGCTCT NGG 10 8 17 8 24 0 100 5 10
    240613180242_pb9p GGTGAAGAGCCAAAGACTCAGAGTAGTTTAGG(+AGGACA)GTACCTTCCCAAACTCCGGGAAGTCCCAAGA NGG 10 8 17 8 24 0 100 5 10
    240613180240_ppin TTGGTGCTGTTGGTCTTTATGGGCTTGAACTG(+AGGACA)GTACCTCACTGTGGGTTTGATTGGCACTTCC NGG 10 8 17 8 24 0 100 5 10
    240613180239_9yfq ATCAAGGAAGTGCCAATCAAACCCACAGTGAG(+AGGACA)GTACCAGTTCAAGCCCATAAAGACCAACAGC NGG 10 8 17 8 24 0 100 5 10
    240613180237_ei2w GGATAAAGCCATTCCCATCTACTGGGAGGGGG(+AGGACA)GTACCTTCTGAGGGGACCAGTCACGCTCACA NGG 10 8 17 8 24 0 100 5 10
    240613180235_anoy CCCACTGTGAGCGTGACTGGTCCCCTCAGAAG(+AGGACA)GTACCCCCCTCCCAGTAGATGGGAATGGCTT NGG 10 8 17 8 24 0 100 5 10