Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613180144_8kx7 TTCAAATGAGCTGTATAGAAAGACACCTTGTG(+AGGACA)GTACCTAAAGTGGAACTGCTTCTTCTGTTTC NGG 10 8 17 8 24 0 100 5 10
    240613180143_g9zw TCACTGAAACAGAAGAAGCAGTTCCACTTTAG(+AGGACA)GTACCACAAGGTGTCTTTCTATACAGCTCAT NGG 10 8 17 8 24 0 100 5 10
    240613180142_sdda CAGACCCCAGTATATTATTTGATCTTATCTTG(+AGGACA)GATCCTTTTGAAAAGTTAAGAATATATGAAG NGG 10 8 17 8 24 0 100 5 10
    240613180141_351e TTTACCTTCATATATTCTTAACTTTTCAAAAG(+AGGACA)GATCCAAGATAAGATCAAATAATATACTGGG NGG 10 8 17 8 24 0 100 5 10
    240613180139_u55z TACAGACCTGACTTTCATAGTCAGGAGCTTGG(+AGGACA)GTACCTGCTTCAGCATCATGGACACTGGGAT NGG 10 8 17 8 24 0 100 5 10
    240613180138_yzu5 CTGGAATCCCAGTGTCCATGATGCTGAAGCAG(+AGGACA)GTACCCAAGCTCCTGACTATGAAAGTCAGGT NGG 10 8 17 8 24 0 100 5 10
    240613180137_yt1v AAGCTCATGTTCTGACTGCAAGGATCATGTTG(+AGGACA)GTACCTACCCTTTAGGAAACTTGCCAGTCAG NGG 10 8 17 8 24 0 100 5 10
    240613180135_zgr7 AATTTCTGACTGGCAAGTTTCCTAAAGGGTAG(+AGGACA)GTACCAACATGATCCTTGCAGTCAGAACATG NGG 10 8 17 8 24 0 100 5 10
    240613180134_nq2b CTTGGGTCTAGGGTGAATTCATCTTCTGCTAG(+AGGACA)GTACCCATGGCCTGCAAGTAGATGCCAGGCA NGG 10 8 17 8 24 0 100 5 10
    240613180132_3qzq AGCCCTGCCTGGCATCTACTTGCAGGCCATGG(+AGGACA)GTACCTAGCAGAAGATGAATTCACCCTAGAC NGG 10 8 17 8 24 0 100 5 10