Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613180315_63t3 TCAAATAACCAGTTAGGAAATATTCCAGGTGG(+AGGACA)GTACCAGGTGGGCTAACGTGTGTATCCCATG NGG 10 8 17 8 24 0 100 5 10
    240613180313_m90i TATGAAGCTCTTCCACTTCCATCCAGGTAAGG(+AGGACA)GATCCAATTGACAGGCAATCTGGTAATACAT NGG 10 8 17 8 24 0 100 5 10
    240613180312_veq1 TTTCAATGTATTACCAGATTGCCTGTCAATTG(+AGGACA)GATCCCTTACCTGGATGGAAGTGGAAGAGCT NGG 10 8 17 8 24 0 100 5 10
    240613180310_cyjv CCAGGGGATCCAACATTTTCTGATCTCTATGG(+AGGACA)GTACCACGCAGGCAAAACACTCATATACATT NGG 10 8 17 8 24 0 100 5 10
    240613180309_26em TATTTAATGTATATGAGTGTTTTGCCTGCGTG(+AGGACA)GTACCCATAGAGATCAGAAAATGTTGGATCC NGG 10 8 17 8 24 0 100 5 10
    240613180305_06gb GCTCACAACCATCTGTAACTCCAGTTCCAGGG(+AGGACA)GATCCAACATTTTCTGATCTCTATGGGTACC NGG 10 8 17 8 24 0 100 5 10
    240613180304_ke4k TGCGTGGTACCCATAGAGATCAGAAAATGTTG(+AGGACA)GATCCCCTGGAACTGGAGTTACAGATGGTTG NGG 10 8 17 8 24 0 100 5 10
    240613180303_ovwm TTAGCTCCTAATATGTCCCTTCTCTCACACTG(+AGGACA)GTACCTCAATTTATGAGTTAAAACTGGCTCT NGG 10 8 17 8 24 0 100 5 10
    240613180301_8p5c TTTAGAGAGCCAGTTTTAACTCATAAATTGAG(+AGGACA)GTACCAGTGTGAGAGAAGGGACATATTAGGA NGG 10 8 17 8 24 0 100 5 10
    240613180259_6mfr GCAGATGGCGTTAAGTGATGTTACTGTCCTGG(+AGGACA)GTACCTAGAGCCTTTAGTTCATGACACGCTG NGG 10 8 17 8 24 0 100 5 10