Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613180116_o90k AGACCCTATACAAGCAGCATCGCTGTTGCCAG(+AGGACA)GATCCTCTTCCTAATGAAGGGTCGCAGAGAC NGG 10 8 17 8 24 0 100 5 10
    240613180115_tket ACAGCGTCTCTGCGACCCTTCATTAGGAAGAG(+AGGACA)GATCCTGGCAACAGCGATGCTGCTTGTATAG NGG 10 8 17 8 24 0 100 5 10
    240613180113_b97d CAGTATTACCATTATAAATGCTATCCTCCAGG(+AGGACA)GATCCGCTGCTCCCACTAGCATAAGCCTTCA NGG 10 8 17 8 24 0 100 5 10
    240613180111_w0cw CAAAGTGAAGGCTTATGCTAGTGGGAGCAGCG(+AGGACA)GATCCCTGGAGGATAGCATTTATAATGGTAA NGG 10 8 17 8 24 0 100 5 10
    240613180110_64yj AGTCCTCTGGAAATTCAGATGAAAGGCGTGAG(+AGGACA)GTACCAAAAATAATAATTTGTCAATTTAGTT NGG 10 8 17 8 24 0 100 5 10
    240613180109_q2x0 TCCACAACTAAATTGACAAATTATTATTTTTG(+AGGACA)GTACCTCACGCCTTTCATCTGAATTTCCAGA NGG 10 8 17 8 24 0 100 5 10
    240613180107_kxe0 GCACTATGTTGACCAAGCCTGCCAGCCCTATG(+AGGACA)GTACCTTTCTTTAAATGGCCGAATCCCTGGT NGG 10 8 17 8 24 0 100 5 10
    240613180105_5nz4 TACTAACCAGGGATTCGGCCATTTAAAGAAAG(+AGGACA)GTACCATAGGGCTGGCAGGCTTGGTCAACAT NGG 10 8 17 8 24 0 100 5 10
    240613180104_d9lm TGCAGCAAGTCATCTAATGCATGTGCACTTAG(+AGGACA)GATCCAAGGACAAGAAAAGAACAGGAGAGAG NGG 10 8 17 8 24 0 100 5 10
    240613180102_b5n9 TGTTTCTCTCTCCTGTTCTTTTCTTGTCCTTG(+AGGACA)GATCCTAAGTGCACATGCATTAGATGACTTG NGG 10 8 17 8 24 0 100 5 10