Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613180216_xs0c TTCTAGGCACACTCCTTGCAGTAGGCTCCAGG(+AGGACA)GTACCTTGGAACTGCAACCAAGGCATGGTAA NGG 10 8 17 8 24 0 100 5 10
    240613180214_75y1 ATTCCTTACCATGCCTTGGTTGCAGTTCCAAG(+AGGACA)GTACCCTGGAGCCTACTGCAAGGAGTGTGCC NGG 10 8 17 8 24 0 100 5 10
    240613180213_d3em GTCTTCTCTATTTGTCTATTAATTGATATCTG(+AGGACA)GATCCCCATGAAATTTTGAGAAAGCTACAGA NGG 10 8 17 8 24 0 100 5 10
    240613180211_6a24 GCAATTCTGTAGCTTTCTCAAAATTTCATGGG(+AGGACA)GATCCAGATATCAATTAATAGACAAATAGAG NGG 10 8 17 8 24 0 100 5 10
    240613180210_wxhd TTCCCTAAGGGAACTTTGAAGTTCATGTGTTG(+AGGACA)GATCCTTCAACACATGGTAAGCACTGCAGGT NGG 10 8 17 8 24 0 100 5 10
    240613180209_n6v9 GCTCCACCTGCAGTGCTTACCATGTGTTGAAG(+AGGACA)GATCCAACACATGAACTTCAAAGTTCCCTTA NGG 10 8 17 8 24 0 100 5 10
    240613180206_ubuj ACATGGAAAAGGACAAAGAGACCATACCTGGG(+AGGACA)GATCCATCCCATTTACAGCCACTAAACCTGG NGG 10 8 17 8 24 0 100 5 10
    240613180205_5msr AGCATCCAGGTTTAGTGGCTGTAAATGGGATG(+AGGACA)GATCCCCAGGTATGGTCTCTTTGTCCTTTTC NGG 10 8 17 8 24 0 100 5 10
    240613180203_157e TTATAGAACCCAGGACCACCAGCCCAGAGATG(+AGGACA)GTACCACCCACAAGGGGCCTTTCCTCCTTGA NGG 10 8 17 8 24 0 100 5 10
    240613180202_fk9o AGTGATCAAGGAGGAAAGGCCCCTTGTGGGTG(+AGGACA)GTACCATCTCTGGGCTGGTGGTCCTGGGTTC NGG 10 8 17 8 24 0 100 5 10