Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613180200_o1bn CTTTATTCCGCCGTGTTGCTGTTTTCTCTCAG(+AGGACA)GATCCTTTGAAGGAAGAAGCTCTTCAGCAAA NGG 10 8 17 8 24 0 100 5 10
    240613180159_3v6o CTGGCTTTGCTGAAGAGCTTCTTCCTTCAAAG(+AGGACA)GATCCTGAGAGAAAACAGCAACACGGCGGAA NGG 10 8 17 8 24 0 100 5 10
    240613180157_iguc GAATAGTTACAAGATGATTTGTGGTATCATGG(+AGGACA)GTACCCATTATAAACCATGCTCTTCCCAGTA NGG 10 8 17 8 24 0 100 5 10
    240613180156_k7kt TCAGCTACTGGGAAGAGCATGGTTTATAATGG(+AGGACA)GTACCCATGATACCACAAATCATCTTGTAAC NGG 10 8 17 8 24 0 100 5 10
    240613180154_x10m AGTGAAAAATGATCTTGTTTTTCTTACAGCCG(+AGGACA)GATCCAAAGACGCTAGATGTTAAGGGCTGAG NGG 10 8 17 8 24 0 100 5 10
    240613180152_k4ct TCAGCCTCAGCCCTTAACATCTAGCGTCTTTG(+AGGACA)GATCCGGCTGTAAGAAAAACAAGATCATTTT NGG 10 8 17 8 24 0 100 5 10
    240613180150_6e8v GTGGTGGTTGGAAGATCTAAATGGGATTCGGG(+AGGACA)GTACCCCCAAGCATGCACCCCCCTATTGCAG NGG 10 8 17 8 24 0 100 5 10
    240613180148_4mkt ACTCACTGCAATAGGGGGGTGCATGCTTGGGG(+AGGACA)GTACCCCGAATCCCATTTAGATCTTCCAACC NGG 10 8 17 8 24 0 100 5 10
    240613180147_553c AAGGGTTTCCACTTGGGCTGGACCCAGGTTTG(+AGGACA)GTACCTAGAAGATCCTTCTGCAGACACCGAG NGG 10 8 17 8 24 0 100 5 10
    240613180146_m32g AGGTCCTCGGTGTCTGCAGAAGGATCTTCTAG(+AGGACA)GTACCAAACCTGGGTCCAGCCCAAGTGGAAA NGG 10 8 17 8 24 0 100 5 10