Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613180401_9ves GTGCTCTTCCATCAAGGCAGACGGGATCAGAG(+AGGACA)GATCCCCATCAGCCCCACCTAGCTCTGAATT NGG 10 8 17 8 24 0 100 5 10
    240613180400_prtc AAAAGAATATACCTTCTTCTCAGCACCTCATG(+AGGACA)GTACCTTCTCCAAAATTGACCATATAATCGG NGG 10 8 17 8 24 0 100 5 10
    240613180359_qxi5 TGTGACCGATTATATGGTCAATTTTGGAGAAG(+AGGACA)GTACCATGAGGTGCTGAGAAGAAGGTATATT NGG 10 8 17 8 24 0 100 5 10
    240613180357_4jjw CCTCTTCCAAACGACCCTAGTTCAATTTCCAG(+AGGACA)GATCCACATGGCAACTCATAGATGTCTGTAA NGG 10 8 17 8 24 0 100 5 10
    240613180355_0wf1 TGAAGTTACAGACATCTATGAGTTGCCATGTG(+AGGACA)GATCCTGGAAATTGAACTAGGGTCGTTTGGA NGG 10 8 17 8 24 0 100 5 10
    240613180354_yzf2 TCAGGGCAGAGAAACTGAGGAGCCTGGAGAAG(+AGGACA)GATCCCTCCGGTTCCCATCTGTGCCTGGAGC NGG 10 8 17 8 24 0 100 5 10
    240613180352_3292 GGTCAGCTCCAGGCACAGATGGGAACCGGAGG(+AGGACA)GATCCTTCTCCAGGCTCCTCAGTTTCTCTGC NGG 10 8 17 8 24 0 100 5 10
    240613180350_78kq ACCACATGGTGGCTCACAACCATCTGTAATAG(+AGGACA)GATCCAATGCACTTTTCTGGTGTGTGTCTGA NGG 10 8 17 8 24 0 100 5 10
    240613180349_d7ny TGTCTTCAGACACACACCAGAAAAGTGCATTG(+AGGACA)GATCCTATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240613180347_35fr ATCACACCACCATTTGTGCCTCCATCCCAGTG(+AGGACA)GATCCAACACCAACCTCTGACCTCCACAGGA NGG 10 8 17 8 24 0 100 5 10