Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613180338_c3i0 ACAGCATTCCGAGTACATGGACACTACATACG(+AGGACA)GTACCAGCTCCCTGGAAAAGATAAATATTGG NGG 10 8 17 8 24 0 100 5 10
    240613180337_606r TCGATCCAATATTTATCTTTTCCAGGGAGCTG(+AGGACA)GTACCGTATGTAGTGTCCATGTACTCGGAAT NGG 10 8 17 8 24 0 100 5 10
    240613180335_81pl AGTAAGGAGGGGACTGAGTACGTAACCTGTTG(+AGGACA)GTACCCTTAAACTGGACAAACGTCACAATGC NGG 10 8 17 8 24 0 100 5 10
    240613180334_3sks ATGTGGCATTGTGACGTTTGTCCAGTTTAAGG(+AGGACA)GTACCAACAGGTTACGTACTCAGTCCCCTCC NGG 10 8 17 8 24 0 100 5 10
    240613180332_t12f TACTGACCAGTTCAGATGGGTCCACTAGAGGG(+AGGACA)GATCCTTGAGCATGCACACTTGGAAAAACAC NGG 10 8 17 8 24 0 100 5 10
    240613180331_sjk3 CATGGGTGTTTTTCCAAGTGTGCATGCTCAAG(+AGGACA)GATCCCCTCTAGTGGACCCATCTGAACTGGT NGG 10 8 17 8 24 0 100 5 10
    240613180329_cth6 TGAGCTTAGCTTGTGCTGGCGGCTGAACTCAG(+AGGACA)GATCCTGTCCTGCACAGGCTAGGCAGGCCCT NGG 10 8 17 8 24 0 100 5 10
    240613180328_tn2f GGTAGAGGGCCTGCCTAGCCTGTGCAGGACAG(+AGGACA)GATCCTGAGTTCAGCCGCCAGCACAAGCTAA NGG 10 8 17 8 24 0 100 5 10
    240613180327_bf0a TAATACCAACATTCACATACTGTATTACAATG(+AGGACA)GTACCACAATATAAAATAAAATATGTATAAA NGG 10 8 17 8 24 0 100 5 10
    240613180326_uo5u GTCAGTTTATACATATTTTATTTTATATTGTG(+AGGACA)GTACCATTGTAATACAGTATGTGAATGTTGG NGG 10 8 17 8 24 0 100 5 10