Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613180409_4nj6 TACAAACACTAAGCATAAAAGCACATGTGGTG(+AGGACA)GTACCTGGGTATCTAGTTCTTACGGAATAAT NGG 10 8 17 8 24 0 100 5 10
    240613180407_bu42 TTGAGATTATTCCGTAAGAACTAGATACCCAG(+AGGACA)GTACCACCACATGTGCTTTTATGCTTAGTGT NGG 10 8 17 8 24 0 100 5 10
    240613180405_jht2 GAAGAAGAATCTTGAAAGGGAGGGTGGTGATG(+AGGACA)GTACCGATAAATACCTGTGTTTTAAAATCTT NGG 10 8 17 8 24 0 100 5 10
    240613180404_3m41 TGTACAAGATTTTAAAACACAGGTATTTATCG(+AGGACA)GTACCATCACCACCCTCCCTTTCAAGATTCT NGG 10 8 17 8 24 0 100 5 10
    240613180402_zv7i TTTAGAATTCAGAGCTAGGTGGGGCTGATGGG(+AGGACA)GATCCTCTGATCCCGTCTGCCTTGATGGAAG NGG 10 8 17 8 24 0 100 5 10
    240613180401_9ves GTGCTCTTCCATCAAGGCAGACGGGATCAGAG(+AGGACA)GATCCCCATCAGCCCCACCTAGCTCTGAATT NGG 10 8 17 8 24 0 100 5 10
    240613180400_prtc AAAAGAATATACCTTCTTCTCAGCACCTCATG(+AGGACA)GTACCTTCTCCAAAATTGACCATATAATCGG NGG 10 8 17 8 24 0 100 5 10
    240613180359_qxi5 TGTGACCGATTATATGGTCAATTTTGGAGAAG(+AGGACA)GTACCATGAGGTGCTGAGAAGAAGGTATATT NGG 10 8 17 8 24 0 100 5 10
    240613180357_4jjw CCTCTTCCAAACGACCCTAGTTCAATTTCCAG(+AGGACA)GATCCACATGGCAACTCATAGATGTCTGTAA NGG 10 8 17 8 24 0 100 5 10
    240613180355_0wf1 TGAAGTTACAGACATCTATGAGTTGCCATGTG(+AGGACA)GATCCTGGAAATTGAACTAGGGTCGTTTGGA NGG 10 8 17 8 24 0 100 5 10