Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613180739_y8uq GTCTCAGGCTCTGGAAGGAAAAGAGAAGACTG(+AGGACA)GTACCAAACCATGCTTTGGGGGAAAGCTGAA NGG 10 8 17 8 24 0 100 5 10
    240613180737_c0ym TTCAGTTCAGCTTTCCCCCAAAGCATGGTTTG(+AGGACA)GTACCAGTCTTCTCTTTTCCTTCCAGAGCCT NGG 10 8 17 8 24 0 100 5 10
    240613180735_gwjt GCCTCCACCTCCAAGTGCTGGATTTACGGAGG(+AGGACA)GATCCAACCTAAGGCCTCTAACAAACACACA NGG 10 8 17 8 24 0 100 5 10
    240613180734_ggq1 GTGCTTGTGTGTTTGTTAGAGGCCTTAGGTTG(+AGGACA)GATCCCTCCGTAAATCCAGCACTTGGAGGTG NGG 10 8 17 8 24 0 100 5 10
    240613180732_8e2l GTCTCAGGCTCTGGAAGGAAAAGAGAAGACTG(+AGGACA)GTACCAAACCATGCTTTGGGGGAAAGCTGAA NGG 10 8 17 8 24 0 100 5 10
    240613180731_49b0 TTCAGTTCAGCTTTCCCCCAAAGCATGGTTTG(+AGGACA)GTACCAGTCTTCTCTTTTCCTTCCAGAGCCT NGG 10 8 17 8 24 0 100 5 10
    240613180730_1vir CAGCAAACTTGACCCCTGATTTTAACCCTCAG(+AGGACA)GATCCACACGGAAGGGGAAAACTCACTCCTG NGG 10 8 17 8 24 0 100 5 10
    240613180728_dky7 ACTTTCAGGAGTGAGTTTTCCCCTTCCGTGTG(+AGGACA)GATCCTGAGGGTTAAAATCAGGGGTCAAGTT NGG 10 8 17 8 24 0 100 5 10
    240613180726_itja CAGGGATCTTCAAGGACAGGCCTGGGTTTGGG(+AGGACA)GATCCCACCTCTTGAGTATGAAAGAATTCAG NGG 10 8 17 8 24 0 100 5 10
    240613180725_9g3d CACTCCTGAATTCTTTCATACTCAAGAGGTGG(+AGGACA)GATCCCCAAACCCAGGCCTGTCCTTGAAGAT NGG 10 8 17 8 24 0 100 5 10