Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613180841_wcqa ACAGCCGCATTCCAGGGTGGGAGAGCGTGCTG(+AGGACA)GTACCATCTTCTCTCAAGTAGCTCTGTCATC NGG 10 8 17 8 24 0 100 5 10
    240613180840_uw1l GCCCCGATGACAGAGCTACTTGAGAGAAGATG(+AGGACA)GTACCAGCACGCTCTCCCACCCTGGAATGCG NGG 10 8 17 8 24 0 100 5 10
    240613180838_q9bc CTCACAAAATCTGATGACATTTTGTGAAGTTG(+AGGACA)GTACCATCTCTACATGCGCCTAAGTGACAGG NGG 10 8 17 8 24 0 100 5 10
    240613180837_lk47 ATGCCCCTGTCACTTAGGCGCATGTAGAGATG(+AGGACA)GTACCAACTTCACAAAATGTCATCAGATTTT NGG 10 8 17 8 24 0 100 5 10
    240613180835_1w50 CATTCATGTAGCATATGCCTCCATTTTTCCTG(+AGGACA)GTACCTAGCATCAGAAGTAACATTTATGAAC NGG 10 8 17 8 24 0 100 5 10
    240613180834_i3yp AGAGAGTTCATAAATGTTACTTCTGATGCTAG(+AGGACA)GTACCAGGAAAAATGGAGGCATATGCTACAT NGG 10 8 17 8 24 0 100 5 10
    240613180833_zq5y TGGGCTCCCAAGCTTGTCTTTGGGTAGAGATG(+AGGACA)GATCCAGTCAGTGTTAGGACACTGGGGAAGC NGG 10 8 17 8 24 0 100 5 10
    240613180831_vniq TATCTGCTTCCCCAGTGTCCTAACACTGACTG(+AGGACA)GATCCATCTCTACCCAAAGACAAGCTTGGGA NGG 10 8 17 8 24 0 100 5 10
    240613180830_saak TTTGGCTGTAGCTTCAGTTCCACTCTGAACTG(+AGGACA)GATCCTCTTGGTAGCTGGACTTAATAAAATG NGG 10 8 17 8 24 0 100 5 10
    240613180829_348r CACCTCATTTTATTAAGTCCAGCTACCAAGAG(+AGGACA)GATCCAGTTCAGAGTGGAACTGAAGCTACAG NGG 10 8 17 8 24 0 100 5 10